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AB193709

Human MBL ELISA Kit (Mannose-Binding Lectin)

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(1 Publication)

Human MBL ELISA Kit (Mannose-Binding Lectin) is a Sandwich (quantitative) ELISA for the measurement of Human MBL (Mannose-Binding Lectin) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

COLEC1, MBL, MBL2, Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin

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Sandwich ELISA - Human MBL ELISA Kit (Mannose-Binding Lectin) (AB193709)
  • sELISA

Supplier Data

Sandwich ELISA - Human MBL ELISA Kit (Mannose-Binding Lectin) (AB193709)

Typical standard curve in Assay Diluent A.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Sandwich ELISA - Human MBL ELISA Kit (Mannose-Binding Lectin) (AB193709)
  • sELISA

Supplier Data

Sandwich ELISA - Human MBL ELISA Kit (Mannose-Binding Lectin) (AB193709)

Typical standard curve in Assay Diluent B.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.03 ng/mL

Range

0.03 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MBL (Mannose-Binding Lectin) Human ELISA Kit (ab193709) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MBL in sera, plasma and cell culture supernatants.

This assay employs an antibody specific for Human MBL coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures MBL present in the samples. The wells are washed and biotinylated anti-Human MBL antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of MBL bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 147 %", "average": "= 122.8" }, { "sample": "Plasma", "range": "98 - 145 %", "average": "= 121.1" }, { "sample": "Cell culture media", "range": "91 - 116 %", "average": "= 103.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193709-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193709-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193709-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193709-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193709-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB193709-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human MBL Microplate (12 strips x 8 wells)", "number":"AB193709-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193709-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human MBL detection antibody", "number":"AB193709-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MBL Standards (lyophilized)", "number":"AB193709-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.
Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.
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Product protocols

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Target data

Calcium-dependent lectin involved in innate immune defense (PubMed : 35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed : 35102342).
See full target information MBL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70089 PubMed40545963

2025

Extracellular Vesicle Proteome Analysis Improves Diagnosis of Recurrence in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Yong Hyon,Min Woo Kim,Kyung-A Hyun,Yeji Yang,Seongmin Ha,Jee Ye Kim,Young Kim,Sunyoung Park,Hogyeong Gawk,Heaji Lee,Suji Lee,Sol Moon,Eun Hee Han,Jin Young Kim,Ji Yeong Yang,Hyo-Il Jung,Seung Il Kim,Young-Ho Chung
View all publications
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