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AB313991

Human MCEMP1 ELISA Kit

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Human MCEMP1 ELISA Kit is a Sandwich ELISA for the measurement of Human MCEMP1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

C19orf59, MCEMP1, Mast cell-expressed membrane protein 1

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Sandwich ELISA - Human MCEMP1 ELISA Kit (AB313991)
  • sELISA

Supplier Data

Sandwich ELISA - Human MCEMP1 ELISA Kit (AB313991)

Example of standard curve using ab313991. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.045 ng/mL

Range

0.045 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MCEMP1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MCEMP1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human MCEMP1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human C1qTNF9, Carbonic Anhydrase VA, CANT1, Cathepsin H, Contactin-5, Chymotrypsin C/CTRC, Draxin, EphB2, EphB3, FABP8, Fgr, FKBP51, FUCA1, Galanin, GALNT10, Gastrokine 1, Glyoxalase II, HS3ST1, HS3ST3B1, Lin28, Lysyl Oxidase Homolog, LRRTM4, Methionine Aminopeptidase 1D, Matrilin-2, Mcl-1, MDGA2, MEF2C, Methionine Aminopeptidase 2, Neurocan, Nogo-A, PCK1, PlGF-2, PON1, SALM4/LRFN3, Semaphorin 6C, SorCS2, ST3GAL1, ST8SIA1, Tsukushi/TSK

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Recovery

[ { "sample": "Serum", "range": "103 - 123 %", "average": "= 111.3" }, { "sample": "Plasma", "range": "98 - 110 %", "average": "= 103.7" }, { "sample": "Cell culture media", "range": "94 - 113 %", "average": "= 100" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313991-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313991-CMP05" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313991-CMP07" }, { "size":"1 x 1 Unit", "name":"Human MCEMP1 Antibody-coated ELISA Plate", "number":"AB313991-CMP08" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human MCEMP1 Detection Antibody", "number":"AB313991-CMP09" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human MCEMP1 Protein Standard", "number":"AB313991-CMP10" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313991-CMP06" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313991-CMP04" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313991-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313991-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313991-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB313991-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB313991-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313991-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313991-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313991-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313991-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human MCEMP1 Antibody-coated ELISA Plate", "number":"AB313991-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human MCEMP1 Detection Antibody", "number":"AB313991-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human MCEMP1 Protein Standard", "number":"AB313991-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCEMP1 also known as Mast Cell Expressed Membrane Protein 1 is a protein that plays an important role in cellular functions. The protein has a molecular mass of approximately 17 kDa. MCEMP1 is mainly expressed on the cell membranes of mast cells which are involved in immune system responses. This expression pattern suggests MCEMP1's involvement in processes closely tied to mast cell biology.
Biological function summary

This protein has significant roles in mast cell activation and signal transduction. MCEMP1 is not part of a known protein complex but it influences mast cell degranulation where cells release histamine and other mediators. This activity places MCEMP1 as an influential protein impacting the body's response to allergens and infections.

Pathways

MCEMP1 is important for pathways related to immune system signaling and allergic responses. In the immune signaling pathway MCEMP1 impacts mast cell mediator release interacting with proteins such as FcεRI a high-affinity receptor for IgE. Another related pathway involves inflammatory responses where MCEMP1 further regulates signals that contribute to the body's defense mechanism against pathogens.

MCEMP1 is linked to allergic reactions and asthma. These conditions often result from excessive activation of mast cells where MCEMP1 plays a regulatory function. The protein interacts closely with FcεRI in asthma a chronic condition characterized by airway inflammation. Understanding the role of MCEMP1 in these disorders helps in exploring potential therapeutic targets for managing allergic conditions.
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Product protocols

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Target data

See full target information MCEMP1
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websiteProtocolBooklet
en
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Product promise

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