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AB314740

Human MCL1 ELISA Kit

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Human MCL1 ELISA Kit is a Sandwich ELISA for the measurement of Human MCL1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3

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Sandwich ELISA - Human MCL1 ELISA Kit (AB314740)
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Supplier Data

Sandwich ELISA - Human MCL1 ELISA Kit (AB314740)

Example of standard curve using ab314740. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.13 ng/mL

Range

0.13 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB32087

Anti-MCL1 antibody [Y37]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (AB32087)

  • 5
  • 14
Proteins & Peptides

AB131682

Recombinant Human MCL1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human MCL1 protein (His tag N-Terminus) (AB131682)

  • 0
  • 0
Cell Lines & Lysates

AB266838

Human MCL1 knockout HEK-293T cell line

Advanced Validation

Western blot - Human MCL1 knockout HEK-293T cell line (AB266838)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MCL1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MCL1 in serum (human MCL1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human MCL1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human C1qTNF9, Carbonic Anhydrase VA, CANT1, Cathepsin H, Contactin-5, Chymotrypsin C/CTRC, Draxin, EphB2, EphB3, FABP8, Fgr, FKBP51, FUCA1, Galanin, GALNT10, Gastrokine 1, Glyoxalase II, HS3ST1, HS3ST3B1, Lin28, Lysyl Oxidase Homolog, LRRTM4, Methionine Aminopeptidase 1D, Matrilin-2, MCEMP1, MDGA2, MEF2C, Methionine Aminopeptidase 2, Neurocan, Nogo-A, PCK1, PlGF-2, PON1, SALM4/LRFN3, Semaphorin 6C, SorCS2, ST3GAL1, ST8SIA1, Tsukushi/TSK.

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Recovery

[ { "sample": "Serum", "range": "75 - 86 %", "average": "= 81.27" }, { "sample": "Plasma", "range": "68 - 78 %", "average": "= 70.46" }, { "sample": "Cell culture media", "range": "96 - 106 %", "average": "= 99.45" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314740-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human MCL1 Detection Antibody", "number":"AB314740-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314740-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314740-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314740-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human MCL1 Protein Standard", "number":"AB314740-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314740-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human MCL1 Antibody-coated ELISA Plate", "number":"AB314740-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314740-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCL1 also known as myeloid cell leukemia sequence 1 is an anti-apoptotic member of the BCL-2 family. This protein plays an important role in regulating cell survival by inhibiting apoptosis. MCL1 has a molecular weight of approximately 37 kDa. It is primarily expressed in the mitochondria and can be found in various tissues including heart liver placenta and kidneys.
Biological function summary

MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.

Pathways

MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.

Researchers have linked alterations in MCL1 expression to various cancers including leukemia and lymphoma. Overexpression of MCL1 can contribute to chemoresistance in cancer cells by preventing apoptosis. This protein's function is also connected to cardiomyopathies where altered MCL1 expression in cardiac tissue can influence cell death and survival outcomes. BCL-2 and BCL-xL closely related proteins also implicate in these conditions highlighting the central role of the apoptotic regulatory network in disease development.
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Product protocols

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Target data

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
See full target information MCL1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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