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Human MCL1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MCL1 in Human in Serum, Plasma, Cell culture supernatant samples.

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Images

Sandwich ELISA - Human MCL1 ELISA Kit (AB314740), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.13 - 25 ng/mL

Sensitivity

= 0.13 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
400X HRP-Streptavidin
1 x 200 µL
5X Assay Diluent (Item E2)
1 x 15 mL
Biotinylated Human MCL1 Detection Antibody
2 x 1 Vial
Human MCL1 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human MCL1 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human MCL1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MCL1 in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.13 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.13 ng/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 81.27

Range

75 - 86 %

Sample type

Plasma

Average %

= 70.46

Range

68 - 78 %

Sample type

Cell culture media

Average %

= 99.45

Range

96 - 106 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human MCL1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MCL1 in serum (human MCL1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human MCL1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human C1qTNF9, Carbonic Anhydrase VA, CANT1, Cathepsin H, Contactin-5, Chymotrypsin C/CTRC, Draxin, EphB2, EphB3, FABP8, Fgr, FKBP51, FUCA1, Galanin, GALNT10, Gastrokine 1, Glyoxalase II, HS3ST1, HS3ST3B1, Lin28, Lysyl Oxidase Homolog, LRRTM4, Methionine Aminopeptidase 1D, Matrilin-2, MCEMP1, MDGA2, MEF2C, Methionine Aminopeptidase 2, Neurocan, Nogo-A, PCK1, PlGF-2, PON1, SALM4/LRFN3, Semaphorin 6C, SorCS2, ST3GAL1, ST8SIA1, Tsukushi/TSK.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MCL1 also known as myeloid cell leukemia sequence 1 is an anti-apoptotic member of the BCL-2 family. This protein plays an important role in regulating cell survival by inhibiting apoptosis. MCL1 has a molecular weight of approximately 37 kDa. It is primarily expressed in the mitochondria and can be found in various tissues including heart liver placenta and kidneys.

Biological function summary

MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.

Pathways

MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.

Associated diseases and disorders

Researchers have linked alterations in MCL1 expression to various cancers including leukemia and lymphoma. Overexpression of MCL1 can contribute to chemoresistance in cancer cells by preventing apoptosis. This protein's function is also connected to cardiomyopathies where altered MCL1 expression in cardiac tissue can influence cell death and survival outcomes. BCL-2 and BCL-xL closely related proteins also implicate in these conditions highlighting the central role of the apoptotic regulatory network in disease development.

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1 product image

  • Sandwich ELISA - Human MCL1 ELISA Kit (ab314740), expandable thumbnail

    Sandwich ELISA - Human MCL1 ELISA Kit (ab314740)

    Example of standard curve using ab314740. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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