JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB179886

Human MCP-1 ELISA Kit

Be the first to review this product! Submit a review

|

(36 Publications)

Human MCP-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MCP-1 with a sensitivity of 1.26 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

9 Images
ELISA - Human MCP-1 ELISA Kit (AB179886)
  • ELISA

Supplier Data

ELISA - Human MCP-1 ELISA Kit (AB179886)
Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Lab

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Interpolated concentration of native MCP1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Lab

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Example of human MCP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Unknown

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Interpolated concentrations of native MCP1 in human cerebrospinal fluid, urine, and cell culture supernatant samples.

The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : cerebrospinal fluid (50%)), urine 100%, and PHA stimulated PBMC supernatant 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 371 pg/mL in cerebrospinal fluid, 144 pg/mL in urine, and 70217 pg/mL in PHA stimulated PBMC supernatant.

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native MCP1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Unknown

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Interpolated concentrations of native MCP1 in human serum and plasma samples.

The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 25%, plasma (heparin), 25%, and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 72 pg/mL in serum, 96 pg/mL in plasma (citrate), 101 pg/mL in plasma (heparin), and 88 pg/mL plasma (EDTA).

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Serum from nine individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 113 pg/mL with a range of 58 - 247 pg/mL.

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Example of human MCP1 standard curve in Sample Diluent NS.

The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)
  • sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Example of human MCP1 standard curve in Sample Diluent NS. The MCP1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.26 pg/mL

Range

4.7 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

You may be interested in:

AB316136

Human DAP12 (pY91) + DAP12 Total ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Human MCP-1 ELISA Kit ab179886 is a rapid single-wash 90-min ELISA kit to measure Human MCP-1 in cerebral spinal fluid, urine, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 1.26 pg/mL.

How the assay works

Human MCP-1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

his kit recognizes both native and recombinant human MCP1 protein in serum, plasma, urine, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Human MCP-1 ELISA Kit ab179886 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human MCP-1 ELISA Kit ab179886

Human MCP-1 ELISA Kit ab179886 has been used to assess inflammation in different physio-pathological contexts such as colon cancer(1), atherosclerosis(2), and kidney disease(3).

References:

(1)Bing Liu et al. 2023, PMID: 37153045,
(2)Hongwei Dan et al. 2023, PMID: 37001814,
(3)Leting Zhou et al. 2023, PMID: 37190516

Save your precious samples

Our 384-well Human MCP-1 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Monkey MCP1 Antibody Pair - BSA and Azide free ab241803

Related and recommended products

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Serum", "range": "94 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "90 - 97 %", "average": "= 94" }, { "sample": "Cerebral Spinal Fluid", "range": "102 - 110 %", "average": "= 105" }, { "sample": "Urine", "range": "90 - 94 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": null, "average": "= 91" }, { "sample": "Citrate plasma", "range": "91 - 95 %", "average": "= 92" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab179886-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB179886-CMP12" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB179886-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Capture Antibody", "number":"AB179886-CMP07" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB179886-CMP02" }, { "size":"2 x 1 Vial", "name":"Human MCP-1 Lyophilized Recombinant Protein (ab9670)", "number":"AB179886-CMP06" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB179886-CMP09" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB179886-CMP05" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB179886-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Detector Antibody", "number":"AB179886-CMP08" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB179886-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab179886-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB179886-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB179886-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MCP-1 Lyophilized Recombinant Protein (ab9670)", "number":"AB179886-CMP06", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB179886-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Capture Antibody", "number":"AB179886-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB179886-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB179886-CMP09", "productcode":"" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB179886-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Detector Antibody", "number":"AB179886-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB179886-CMP11", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
style
left-column

Product protocols

style
left-column

Target data

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).
See full target information CCL2
style
left-column

Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Biological procedures online 25:10 PubMed37085762

2023

Exosomal microRNA-342-5p secreted from adipose-derived mesenchymal stem cells mitigates acute kidney injury in sepsis mice by inhibiting TLR9.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Chenghuan Hu,Buyao Zhang,Mingxia Li,Fuxing Deng,Shuangping Zhao

Oncology letters 25:225 PubMed37153045

2023

Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Junmei Li,Feng Yu,Bo Wang,Bing Liu

The Journal of biological chemistry 299:104657 PubMed37001814

2023

miR-147a targets ZEB2 to regulate ox-LDL-induced monocyte adherence to HUVECs, atherosclerotic plaque formation, and stability in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengying Chen,Yuzhen Ning,Jingying Liu,Ming Lian,Juanjuan Wang,Hongwei Dan

Brain sciences 13: PubMed37190516

2023

Association of Urinary Biomarkers of Renal Tubular Injury with Cognitive Dysfunction in Older Patients with Chronic Kidney Disease: A Cross-Sectional Observational Study.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Wang,Ling Hu,Difei Zhou,Xiaolei Chen,Leting Zhou

Proceedings of the National Academy of Sciences of the United States of America 120:e2213584120 PubMed36943879

2023

Influence of glycan structure on the colonization of on human respiratory epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ye-Yu Chun,Kai Sen Tan,Lisa Yu,Michelle Pang,Ming Hui Millie Wong,Rei Nakamoto,Wan-Zhen Chua,Amanda Huee-Ping Wong,Zhe Zhang Ryan Lew,Hsiao Hui Ong,Vincent T Chow,Thai Tran,De Yun Wang,Lok-To Sham

Medicine 102:e32384 PubMed36820569

2023

Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Yang,Youjiang Qiu

Disease markers 2022:4433313 PubMed35692875

2022

Predictive Value of Monocyte Chemoattractant Protein-1 in the Development of Diastolic Dysfunction in Patients with Psoriatic Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Iva Uravić Bursać,Tatjana Kehler,Vedrana Drvar,Emina Babarović,Vesna Pehar Pejčinović,Antonija Ružić Baršić,Viktor Peršić,Gordana Laskarin

Cytokine 153:155868 PubMed35358903

2022

Clinical monitored in subjects metabolically healthy and unhealthy before and during a SARS-CoV-2 infection- A cross-sectional study in Mexican population.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Treviño,Steffany Cortezano-Esteban,Hugo Hernández-Fragoso,Alfonso Díaz,Rubén Vázquez-Roque,Victor Enrique Sarmiento-Ortega,Diana Moroni-González,Rosana Pelayo,Eduardo Brambila

Cytotherapy 24:691-698 PubMed35279374

2022

Assessment of the LOVO device for final harvest of novel cell therapies: a Production Assistance for Cellular Therapies multi-center study.

Applications

Unspecified application

Species

Unspecified reactive species

Laarni Ibenana,Robert Anderson,Adrian Gee,Margaret Gilbert,Cheryl Cox,Joshua M Hare,Adriana Brooks,Linda Kelley,Aisha Khan,Natalia Lapteva,Aaron Orozco,David Styers,Darin Sumstad,Ibekwe Ugochi,David H McKenna

Journal of inflammation research 15:1501-1519 PubMed35261546

2022

Osthole Regulates Secretion of Pro-Inflammatory Cytokines and Expression of and in Normal Human Keratinocytes and Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Kordulewska,Justyna Topa,Anna Cieślińska,Beata Jarmołowska
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column

Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB100586

Human MCP1 ELISA Kit

elisa-kits

human-mcp1-elisa-kit-ab100586

0

(0 reviews)

Alternative Product
Assay Kits

AB214476

Human MCP1 Matched Antibody Pair Kit

matched-antibody-pair-kits

human-mcp1-matched-antibody-pair-kit-ab214476

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com