Human MCP-1 ELISA Kit ab179886 is a single-wash 90-min SimpleStep ELISA used to quantify Human MCP-1 with a sensitivity of 1.26 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human MCP-1 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
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- Biological procedures online 25:102023Exosomal microRNA-342-5p secreted from adipose-derived mesenchymal stem cells mitigates acute kidney injury in sepsis mice by inhibiting TLR9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Liu,Chenghuan Hu,Buyao Zhang,Mingxia Li,Fuxing Deng,Shuangping ZhaoPubMed 37085762
- Oncology letters 25:2252023Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Li,Junmei Li,Feng Yu,Bo Wang,Bing LiuPubMed 37153045
- The Journal of biological chemistry 299:1046572023miR-147a targets ZEB2 to regulate ox-LDL-induced monocyte adherence to HUVECs, atherosclerotic plaque formation, and stability in atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengying Chen,Yuzhen Ning,Jingying Liu,Ming Lian,Juanjuan Wang,Hongwei DanPubMed 37001814
- Brain sciences 13:2023Association of Urinary Biomarkers of Renal Tubular Injury with Cognitive Dysfunction in Older Patients with Chronic Kidney Disease: A Cross-Sectional Observational Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiwei Wang,Ling Hu,Difei Zhou,Xiaolei Chen,Leting ZhouPubMed 37190516
- Proceedings of the National Academy of Sciences of the United States of America 120:e22135841202023Influence of glycan structure on the colonization of on human respiratory epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe-Yu Chun,Kai Sen Tan,Lisa Yu,Michelle Pang,Ming Hui Millie Wong,Rei Nakamoto,Wan-Zhen Chua,Amanda Huee-Ping Wong,Zhe Zhang Ryan Lew,Hsiao Hui Ong,Vincent T Chow,Thai Tran,De Yun Wang,Lok-To ShamPubMed 36943879
- Medicine 102:e323842023Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGui Yang,Youjiang QiuPubMed 36820569
- Disease markers 2022:44333132022Predictive Value of Monocyte Chemoattractant Protein-1 in the Development of Diastolic Dysfunction in Patients with Psoriatic Arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIva Uravić Bursać,Tatjana Kehler,Vedrana Drvar,Emina Babarović,Vesna Pehar Pejčinović,Antonija Ružić Baršić,Viktor Peršić,Gordana LaskarinPubMed 35692875
- Cytokine 153:1558682022Clinical monitored in subjects metabolically healthy and unhealthy before and during a SARS-CoV-2 infection- A cross-sectional study in Mexican population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel Treviño,Steffany Cortezano-Esteban,Hugo Hernández-Fragoso,Alfonso Díaz,Rubén Vázquez-Roque,Victor Enrique Sarmiento-Ortega,Diana Moroni-González,Rosana Pelayo,Eduardo BrambilaPubMed 35358903
- Cytotherapy 24:691-6982022Assessment of the LOVO device for final harvest of novel cell therapies: a Production Assistance for Cellular Therapies multi-center study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaarni Ibenana,Robert Anderson,Adrian Gee,Margaret Gilbert,Cheryl Cox,Joshua M Hare,Adriana Brooks,Linda Kelley,Aisha Khan,Natalia Lapteva,Aaron Orozco,David Styers,Darin Sumstad,Ibekwe Ugochi,David H McKennaPubMed 35279374
- Journal of inflammation research 15:1501-15192022Osthole Regulates Secretion of Pro-Inflammatory Cytokines and Expression of and in Normal Human Keratinocytes and Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Kordulewska,Justyna Topa,Anna Cieślińska,Beata JarmołowskaPubMed 35261546
Key facts
- Detection method
Colorimetric
- Sample types
Cerebral Spinal Fluid, Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
4.7 - 300 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.26 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:9837883, PubMed:10587439, PubMed:10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9837883, PubMed:10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8627182, PubMed:9792674, PubMed:8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).
Alternative names
C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1, MCP1, SCYA2, CCL2
What's included?
Recommended products
Human MCP-1 ELISA Kit ab179886 is a single-wash 90-min SimpleStep ELISA used to quantify Human MCP-1 with a sensitivity of 1.26 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human MCP-1 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
Key facts
- Detection method
Colorimetric
- Sample types
Cerebral Spinal Fluid, Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
4.7 - 300 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.26 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 2.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 6.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 98 | Range 97 - 99 % |
Sample type Serum | Average % = 97 | Range 94 - 101 % |
Sample type EDTA Plasma | Average % = 94 | Range 90 - 97 % |
Sample type Cerebral Spinal Fluid | Average % = 105 | Range 102 - 110 % |
Sample type Urine | Average % = 92 | Range 90 - 94 % |
Sample type Heparin Plasma | Average % = 91 | Range |
Sample type Citrate plasma | Average % = 92 | Range 91 - 95 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human MCP1 (CCL2) ELISA kit (ab179886) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in human serum, plasma, urine, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human MCP1 with 1.26 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human MCP1 protein in serum, plasma, urine, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY This kit recognizes human MCP1 protein.
Other species reactivity was determined by measuring MCP1 (100%) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse and cow.
CROSS REACTIVITY Recombinant mouse MCP1 and rat MCP1 were prepared at 100 pg/mL and 50 pg/mL and assayed for cross reactivity. Cross-reactivity was only observed at 50 ng/mL.
CALIBRATION
This immunoassay is calibrated against a highly purified human MCP1. The NIBSC/WHO unclassified purified human MCP1 preparation 92/794 was evaluated in this kit.
The dose response curve of the unclassified standard MCP1 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep human MCP1 kit to approximate NIBSC 92/794 units, use the equation below.
NIBSC (92/794) approximate value (U/mL) = 0.000266 x SimpleStep human MCP1 value (pg/mL).
MCP-1 (CCL2) is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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6 product images
Sandwich ELISA - Human MCP-1 ELISA Kit (ab179886)
Example of human MCP1 standard curve in Sample Diluent NS.
The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MCP-1 ELISA Kit (ab179886)
Interpolated concentrations of native MCP1 in human serum and plasma samples.
The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 25%, plasma (heparin), 25%, and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 72 pg/mL in serum, 96 pg/mL in plasma (citrate), 101 pg/mL in plasma (heparin), and 88 pg/mL plasma (EDTA).
Sandwich ELISA - Human MCP-1 ELISA Kit (ab179886)
Interpolated concentrations of native MCP1 in human cerebrospinal fluid, urine, and cell culture supernatant samples.
The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cerebrospinal fluid (50%)), urine 100%, and PHA stimulated PBMC supernatant 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 371 pg/mL in cerebrospinal fluid, 144 pg/mL in urine, and 70217 pg/mL in PHA stimulated PBMC supernatant.
Sandwich ELISA - Human MCP-1 ELISA Kit (ab179886)
Serum from nine individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 113 pg/mL with a range of 58 - 247 pg/mL.
Sandwich ELISA - Human MCP-1 ELISA Kit (ab179886)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native MCP1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human MCP-1 ELISA Kit (ab179886)
Example of human MCP1 standard curve in Sample Diluent NS. The MCP1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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