Human MCP1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MCP1 in Human in Plasma, Cell culture supernatant, Serum samples.
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- Communications biology 5:3792022Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmmelie Cansby,Sima Kumari,Mara Caputo,Ying Xia,Rando Porosk,Jonathan Robinson,Hao Wang,Britt-Marie Olsson,Josefine Vallin,Julie Grantham,Ursel Soomets,L Thomas Svensson,Carina Sihlbom,Hanns-Ulrich Marschall,Andreas Edsfeldt,Isabel Goncalves,Margit MahlapuuPubMed 35440683
- NPJ Parkinson's disease 6:212020Cerebrospinal fluid monocyte chemoattractant protein 1 correlates with progression of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Santaella et. al.PubMed 32944649
- Experimental and therapeutic medicine 19:1297-13032020Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wang,Yupei Ke,Tao ShuPubMed 32010302
- International journal of clinical and experimental 12:3320-33282019The upregulation of miR-101 promotes vascular endothelial cell apoptosis and suppresses cell migration in acute coronary syndrome by targeting CDH5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimin Cao et. al.PubMed 31934175
- Molecular medicine reports 20:830-8362019Effect of macrophage migration inhibitory factor on inflammatory cytokines and fibrogenic gene expression in human RPE cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Qin et. al.PubMed 31180524
- Biomedicine & pharmacotherapy = Biomedecine & phar 115:1088762019Salvianolic acid B remits LPS-induced injury by up-regulating miR-142-3p in MH7A cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeqian Meng et. al.PubMed 31022596
- Molecular biology reports 46:1239-12462019Potential role of serum fetuin-A in relation with pro-inflammatory, chemokine and adhesion molecules in diabetic kidney disease: a case-control study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaik Sarfaraz Nawaz et. al.PubMed 30632070
- Life sciences 216:271-2782018RETRACTED: miR-141 alleviates LPS-induced inflammation injury in WI-38 fibroblasts by up-regulation of NOX2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeibei Quan,Huating Zhang,Ruirui XuePubMed 30500550
- Cancer science 110:334-3442018Conophylline suppresses pancreatic cancer desmoplasia and cancer-promoting cytokines produced by cancer-associated fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorihiro Ishii,Kenichiro Araki,Takehiko Yokobori,Kei Hagiwara,Dorgormaa Gantumur,Takahiro Yamanaka,Tadashi Handa,Mariko Tsukagoshi,Takamichi Igarashi,Akira Watanabe,Norio Kubo,Norifumi Harimoto,Atsushi Masamune,Kazuo Umezawa,Hiroyuki Kuwano,Ken ShirabePubMed 30353606
- Iranian journal of basic medical sciences 21:449-4542018The effect of down-regulation of CCL5 on lipopolysaccharide-induced WI-38 fibroblast injury: a potential role for infantile pneumonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Bai,Anbang Han,Shan CongPubMed 29922423
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2 - 500 pg/mL
- Sensitivity
- < 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).
Alternative names
MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1
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Human MCP1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MCP1 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2 - 500 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 2 pg/mL
- General recovery
- 93%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97.62 | Range 85 - 104 % |
Sample type Serum | Average % = 92.65 | Range 80 - 102 % |
Sample type Plasma | Average % = 90.42 | Range 79 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's MCP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human MCP1 ELISA Kit (CCL2) (Human MCP-1 ELISA Kit ab179886) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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3 product images
Sandwich ELISA - Human MCP1 ELISA Kit (ab100586)
Human MCP1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 2-10 fold.
Sandwich ELISA - Human MCP1 ELISA Kit (ab100586)
Human MCP1 standard curve: mean of duplicates (+/- SD)
Sandwich ELISA - Human MCP1 ELISA Kit (ab100586)
Representative Standard Curve using ab100586
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