JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325623

Human MCP1 ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Human MCP1 ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human MCP1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 1.1 - 800 pg/ml with a sensitivity of 0.9 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

6 Images
Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)
  • sELISA

Lab

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)
  • sELISA

Lab

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)

Standard curve comparison between the Chemiluminescent Human MCP1 SimpleStep ELISA and colorimetric Human MCP1 SimpleStep ELISA (ab179886) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)
  • sELISA

Lab

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)

Dynamic range comparison between the Chemiluminescent Human MCP1 SimpleStep ELISA, colorimetric Human MCP1 SimpleStep ELISA (ab179886), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)
  • sELISA

Lab

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)

Interpolated concentrations of native MCP1 in human serum and plasma samples. The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 72 pg/mL in serum, 96 pg/mL in plasma (citrate), 101 pg/mL in plasma (heparin), and 88 pg/mL plasma (EDTA).

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)
  • sELISA

Lab

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)

Interpolated concentrations of native MCP1 in human cerebrospinal fluid, urine, and cell culture supernatant samples. The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : cerebrospinal fluid (50%), urine 100%, and PHA stimulated PBMC supernatant 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 371 pg/mL in cerebrospinal fluid, 144 pg/mL in urine, and 70,217 pg/mL in PHA stimulated PBMC supernatant.

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)
  • sELISA

Lab

Sandwich ELISA - Human MCP1 ELISA Kit, Chemiluminescent (AB325623)

Serum from nine individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 113 pg/mL with a range of 58 - 247 pg/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Cell culture supernatant, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.9 pg/mL

Range

1.1 - 800 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human MCP1 ELISA Kit, Chemiluminescent (ab325623) is a rapid single-wash sandwich 55 min ELISA to measure Human MCP1 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Urine, Cell Culture Supernatant, Cerebrospinal Fluid with a sensitivity of 0.9 pg/mL.

How the assay works

Human MCP1 ELISA Kit, Chemiluminescent (ab325623) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MCP1 (ab325623) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MCP1 antibody [EPR18733-62] - BSA and Azide free (Capture) ab259463
- Anti-MCP1 antibody [EPR18733-74] - BSA and Azide free (Detector) ab259464
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.7" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "94 - 101 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "91 - 95 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "90 - 97 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "91 - 91 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Urine", "range": "90 - 94 %", "average": "= 92" }, { "sample": "Cerebral Spinal Fluid", "range": "102 - 110 %", "average": "= 105" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325623-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human MCP-1 Lyophilized Recombinant Protein (ab9670)", "number":"AB325623-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Capture Antibody", "number":"AB325623-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP1 Detector Antibody", "number":"AB325623-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325623-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325623-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325623-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325623-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325623-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325623-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325623-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).
See full target information CCL2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com