Human MCP2 ELISA Kit
- Recombinant
- SimpleStep
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Human MCP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MCP2 with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
MCP2, SCYA10, SCYA8, CCL8, C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Example of Human MCP2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Interpolated concentrations of native MCP2 in Human 1.5%, 2-day PHA-treated PBMC extract, based on a 50 μg/mL extract load.
The concentrations of MCP2 were measured in duplicate and interpolated from the MCP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 37 pg/ml in treated PBMC extract and undetectable in untreated PBMC extract (not shown).
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Interpolated concentrations of native MCP2 in Human serum and plasma samples.
The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 19 pg/mL in neat serum, 16 pg/mL in neat plasma (citrate), 18 pg/ml in neat plasma (heparin), and 15 pg/mL in neat plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Interpolated concentrations of spike MCP2 in Human serum and plasma samples diluted in Sample Diluent 75BS.
The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Serum from ten individual healthy Human male donors was diluted four-fold and measured in duplicate.
Interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 21 pg/mL with a range of 12-32 pg/mL in neat serum.
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Example of Human MCP2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Interpolated concentrations of native MCP2 in Human 1.5%-PHA-treated and untreated PBMC (2 days) supernatants.
The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC treated supernatant 0.08%, PBMC untreated supernatant 0.08%. The interpolated dilution factor corrected values of treated PBMC supernatants are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 80,341 pg/mL in neat treated PBMC supernatant and undetectable in untreated supernatants (not shown).
- sELISA
Supplier Data
Sandwich ELISA - Human MCP2 ELISA Kit (AB223856)
Example of Human MCP2 standard curve in Sample Diluent 75BS.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human MCP2 ELISA Kit (ab223856) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP2 with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Monocyte chemoattractant protein 2 (MCP2), known also as C-C motif chemokine 8 (CCL8) is chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. Chemokines and their receptors, in conjunction with adhesion molecules and their ligands, provide cues to enable tissue-specific homing of T cells in the steady state and during inflammation. The processed form MCP2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and MCP2. MCP2 protein can bind heparin.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MCP2 influences the migration and activation of leukocytes enhancing the body's immune response. MCP2 interacts with chemokine receptors like CCR2 and CCR5 facilitating the movement of immune cells through tissues. This protein is not usually part of larger complexes but works closely with other chemokines to coordinate cellular responses during inflammation. MCP2 activity is important in processes that require rapid cell recruitment and immune surveillance.
Pathways
MCP2 functions within the chemokine signaling pathway and plays a significant part in the inflammatory response pathway. MCP2 closely interacts with chemokine receptors such as CCR2 which is also engaged by related chemokines like MCP1 (CCL2). MCP2 and MCP1 share functional similarities in modulating the immune response though they target different receptor profiles that expand their influence across numerous immune processes.
Product protocols
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Target data
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