Human MCP2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MCP2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.25 - 160 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
Alternative names
C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2, MCP2, SCYA10, SCYA8, CCL8
What's included?
Recommended products
Human MCP2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MCP2 in Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Alternative names
C-C motif chemokine 8, HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, Small-inducible cytokine A8, MCP-2, MCP2, SCYA10, SCYA8, CCL8
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.25 - 160 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 3 | mean - | SD - | C.V. 3.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 3 | mean - | SD - | C.V. 10.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 132 | Range 123 - 146 % |
Sample type EDTA Plasma | Average % = 94 | Range 85 - 100 % |
Sample type Cell culture extracts | Average % = 99 | Range 84 - 117 % |
Sample type Heparin Plasma | Average % = 113 | Range 112 - 117 % |
Sample type Citrate plasma | Average % = 126 | Range 115 - 138 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human MCP2 ELISA Kit (ab223856) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP2 with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Monocyte chemoattractant protein 2 (MCP2), known also as C-C motif chemokine 8 (CCL8) is chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. Chemokines and their receptors, in conjunction with adhesion molecules and their ligands, provide cues to enable tissue-specific homing of T cells in the steady state and during inflammation. The processed form MCP2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and MCP2. MCP2 protein can bind heparin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP2 also known as CCL8 or monocyte chemoattractant protein-2 plays an important role in leukocyte trafficking. This chemokine has a molecular weight of approximately 8 kDa. CCL8 is expressed in various tissues including macrophages fibroblasts and endothelial cells and it is particularly high during inflammatory responses. The MCP2 protein attracts immune cells such as monocytes and T cells to sites of infection or injury.
- Biological function summary
MCP2 influences the migration and activation of leukocytes enhancing the body's immune response. MCP2 interacts with chemokine receptors like CCR2 and CCR5 facilitating the movement of immune cells through tissues. This protein is not usually part of larger complexes but works closely with other chemokines to coordinate cellular responses during inflammation. MCP2 activity is important in processes that require rapid cell recruitment and immune surveillance.
- Pathways
MCP2 functions within the chemokine signaling pathway and plays a significant part in the inflammatory response pathway. MCP2 closely interacts with chemokine receptors such as CCR2 which is also engaged by related chemokines like MCP1 (CCL2). MCP2 and MCP1 share functional similarities in modulating the immune response though they target different receptor profiles that expand their influence across numerous immune processes.
- Associated diseases and disorders
MCP2 is associated with inflammatory diseases and certain types of cancer. Rheumatoid arthritis shows heightened levels of MCP2 contributing to the persistent inflammation characteristic of the disorder. Elevated MCP2 levels have also been noticed in tumor microenvironments suggesting a role in cancer metastasis where it might interact with proteins such as IL-6 to promote the survival and migration of tumor cells.
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8 product images
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Example of Human MCP2 standard curve in Sample Diluent 75BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Interpolated concentrations of native MCP2 in Human serum and plasma samples.
The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 19 pg/mL in neat serum, 16 pg/mL in neat plasma (citrate), 18 pg/ml in neat plasma (heparin), and 15 pg/mL in neat plasma (EDTA).
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Interpolated concentrations of native MCP2 in Human 1.5%-PHA-treated and untreated PBMC (2 days) supernatants.
The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC treated supernatant 0.08%, PBMC untreated supernatant 0.08%. The interpolated dilution factor corrected values of treated PBMC supernatants are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 80,341 pg/mL in neat treated PBMC supernatant and undetectable in untreated supernatants (not shown).
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Example of Human MCP2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Example of Human MCP2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Interpolated concentrations of spike MCP2 in Human serum and plasma samples diluted in Sample Diluent 75BS.
The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Interpolated concentrations of native MCP2 in Human 1.5%, 2-day PHA-treated PBMC extract, based on a 50 μg/mL extract load.
The concentrations of MCP2 were measured in duplicate and interpolated from the MCP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 37 pg/ml in treated PBMC extract and undetectable in untreated PBMC extract (not shown).
Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
Serum from ten individual healthy Human male donors was diluted four-fold and measured in duplicate.
Interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 21 pg/mL with a range of 12-32 pg/mL in neat serum.
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