AB322362

Human MCP3 (CCL7) ELISA Kit

Recombinant
SimpleStep
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Human MCP3 (CCL7) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MCP3 (CCL7) with a sensitivity of 12.737 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3

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Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

Interpolated concentration of native MCP3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% stimulated PBMC supernatant (1.5% PHA-M stimulation for 46 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

2,800 pg/mL of recombinant human MCP1 (CCL2), MCP2 (CCL8), and Eotaxin (CCL11) were individually tested for interference with 1,400 pg/mL of recombinant human MCP3 (CCL7). Percent reactivity was determined by interpolating the protein concentration from the human MCP3 (CCL7) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No significant interference was observed.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

50 ng/mL of recombinant human MCP1 (CCL2), MCP2 (CCL8), and Eotaxin (CCL11) were individually tested for cross reactivity. Percent reactivity was determined by interpolating the protein concentration from the human MCP3 (CCL7) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No cross reactivity was observed.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

Interpolated concentration of native MCP3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 50% unstimulated PBMC supernatant and 20% stimulated PBMC supernatant (1.5% PHA-M stimulation for 46 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No signal was detected in unstimulated PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

Example of human MCP3 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

Standard curve comparison between the original Human MCP3 SimpleStep ELISA (ab193769) and current Human MCP3 SimpleStep ELISA (ab322362).

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (AB322362)

Example of human MCP3 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 12.737 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MCP3 (CCL7) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP3 (CCL7) protein in Serum, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Human MCP3 (CCL7) with 12.737 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.

See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6" } ]

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Recovery

[ { "sample": "Serum", "range": "119 - 125 %", "average": "= 122" }, { "sample": "Citrate plasma", "range": "89 - 103 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "128 - 135 %", "average": "= 131" }, { "sample": "Cell culture supernatant", "range": "114 - 116 %", "average": "= 115" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

See full target information CCL7

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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