Human MCP3 (CCL7) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MCP3 (CCL7) in Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant samples.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
43.75 - 2800 pg/mL
- Assay time
1h 30m
- Sensitivity
= 12.737 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Alternative names
MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3
What's included?
Human MCP3 (CCL7) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MCP3 (CCL7) in Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant samples.
Key facts
- Detection method
Colorimetric
- Sample types
Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
43.75 - 2800 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 12.737 pg/mL
- General recovery
122%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. = 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. = 6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 122 | Range 119 - 125 % |
Sample type Citrate plasma | Average % = 95 | Range 89 - 103 % |
Sample type EDTA Plasma | Average % = 131 | Range 128 - 135 % |
Sample type Cell culture supernatant | Average % = 115 | Range 114 - 116 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human MCP3 (CCL7) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP3 (CCL7) protein in Serum, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Human MCP3 (CCL7) with 12.737 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (ab322362)
Standard curve comparison between the original Human MCP3 SimpleStep ELISA (Human MCP3 ELISA Kit (CCL7) ab193769) and current Human MCP3 SimpleStep ELISA (ab322362).
Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (ab322362)
2,800 pg/mL of recombinant human MCP1 (CCL2), MCP2 (CCL8), and Eotaxin (CCL11) were individually tested for interference with 1,400 pg/mL of recombinant human MCP3 (CCL7). Percent reactivity was determined by interpolating the protein concentration from the human MCP3 (CCL7) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No significant interference was observed.
Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (ab322362)
50 ng/mL of recombinant human MCP1 (CCL2), MCP2 (CCL8), and Eotaxin (CCL11) were individually tested for cross reactivity. Percent reactivity was determined by interpolating the protein concentration from the human MCP3 (CCL7) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No cross reactivity was observed.
Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (ab322362)
Interpolated concentration of native MCP3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% stimulated PBMC supernatant (1.5% PHA-M stimulation for 46 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (ab322362)
Example of human MCP3 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (ab322362)
Interpolated concentration of native MCP3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: 50% unstimulated PBMC supernatant and 20% stimulated PBMC supernatant (1.5% PHA-M stimulation for 46 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No signal was detected in unstimulated PBMC supernatant.
Sandwich ELISA - Human MCP3 (CCL7) ELISA Kit (ab322362)
Example of human MCP3 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
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