AB193769

Human MCP3 ELISA Kit (CCL7)

SimpleStep
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(3 Publications)

Human MCP3 ELISA Kit (CCL7) is a single-wash 90-min Simplestep used to quantify Human MCP3 (CCL7) with a sensitivity of 10.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3

4 Images

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Titration of 48 hours PHA-stimulated PBMC cell culture supernatant samples within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Example of MCP3 standard curve prepared in Sample Diluent 50 BP.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Comparison of MCP3 concentrations in unstimulated and PHA-stimulated Human PBMC.

PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 48 hours. MCP3 concentrations were measured in 4 X and 8 X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and 10F RPMI1640 media. Raw data values (mean +/-SD, n=2) are graphed. The dotted line represents Blank control.

sELISA

Supplier Data

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Example of MCP3 standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10.3 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MCP3 ELISA Kit (CCL7) (ab193769) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP3 (CCL7) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP3 (CCL7) with 10.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MCP3 (CCL7) is a monomeric secreted chemotactic factor of intercrine (chemokine cc) family. MCP3 attracts monocytes and eosinophils, but not neutrophils. MCP3 augments monocyte anti-tumor activity. It also induces the release of gelatinase B. MCP3 protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]

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Recovery

[ { "sample": "Serum", "range": "98.4 - 101.2 %", "average": "= 99.7" }, { "sample": "EDTA Plasma", "range": "84.7 - 89.6 %", "average": "= 87.8" }, { "sample": "Heparin Plasma", "range": "97 - 99.5 %", "average": "= 98.5" }, { "sample": "Citrate plasma", "range": "95.6 - 101 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "103.8 - 112.9 %", "average": "= 107.3" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target MCP-3 also referred to as CCL7 is a chemotactic cytokine belonging to the CC chemokine family. Its molecular weight is approximately 11 kDa. MCP-3 is largely expressed by various cell types including monocytes lymphocytes and endothelial cells. This protein plays an important role in immune system signaling. MCP-3 exhibits chemotactic activity for monocytes T lymphocytes and natural killer (NK) cells contributing to immune cell recruitment at sites of inflammation.

Biological function summary

MCP-3 interacts with several chemokine receptors such as CCR1 CCR2 and CCR3 allowing for diverse roles in immune response and inflammation. It functions as both a monomer and in conjunction with other chemokines to form heterodimers aiding in the modulation and fine-tuning of the immune response. The presence of MCP-3 in inflammatory tissues indicates its contribution to directing leukocyte migration and positioning therefore supporting both innate and adaptive immunity.

Pathways

MCP-3 is a component of the chemokine signaling pathway and is significant in the regulation of immune surveillance. It interacts closely with proteins such as CCL2 and CCL3 all of which bind to similar receptors like CCR2 and CCR5. MCP-3's involvement in these pathways supports cellular responses to inflammation and infection and its activity has a direct impact on the mobilization and activation of immune cells.

MCP-3 shows associations with conditions marked by inflammation and immune system dysregulation including rheumatoid arthritis and asthma. The protein's influence in these disorders links to other chemokines like MCP-1 (CCL2) which also recruits monocytes and contributes to chronic inflammation. Disruption in MCP-3 expression or function can aggravate disease states by altering the balance of leukocyte trafficking and exacerbating inflammatory responses.

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Product protocols

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Target data

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

See full target information CCL7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 46:102079 PubMed34454163

2021

Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Kong,Wenhui Dong,Yuwen Zhu,Zhiwen Fan,Xiulian Miao,Yan Guo,Chengping Li,Yunfei Duan,Yunjie Lu,Zilong Li,Yong Xu

Journal of cellular physiology 235:10094-10108 PubMed32722876

2020

Helicobacter pylori promote inflammation and host defense through the cagA-dependent activation of mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Guang-Jing Feng,Yi Chen,Ke Li

Toxicological sciences : an official journal of the Society of Toxicology 175:210-219 PubMed32219433

2020

Per- and Polyfluoroalkyl Substances Differentially Inhibit Placental Trophoblast Migration and Invasion In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

John T Szilagyi,Anastasia N Freedman,Stewart L Kepper,Arjun M Keshava,Jackie T Bangma,Rebecca C Fry

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MCP3 ELISA Kit (CCL7)

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise