Human MCP3 ELISA Kit (CCL7) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MCP3 (CCL7) in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Redox biology 46:1020792021Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Kong,Wenhui Dong,Yuwen Zhu,Zhiwen Fan,Xiulian Miao,Yan Guo,Chengping Li,Yunfei Duan,Yunjie Lu,Zilong Li,Yong XuPubMed 34454163
- Journal of cellular physiology 235:10094-101082020Helicobacter pylori promote inflammation and host defense through the cagA-dependent activation of mTORC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang-Jing Feng,Yi Chen,Ke LiPubMed 32722876
- Toxicological sciences : an official journal of the Society of Toxicology 175:210-2192020Per- and Polyfluoroalkyl Substances Differentially Inhibit Placental Trophoblast Migration and Invasion In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn T Szilagyi,Anastasia N Freedman,Stewart L Kepper,Arjun M Keshava,Jackie T Bangma,Rebecca C FryPubMed 32219433
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
46.875 - 3000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 10.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Alternative names
MCP3, SCYA6, SCYA7, CCL7, MCP3, SCYA6, SCYA7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3
What's included?
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Human MCP3 ELISA Kit (CCL7) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MCP3 (CCL7) in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
46.875 - 3000 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 10.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 6.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 99.7 | Range 98.4 - 101.2 % |
Sample type EDTA Plasma | Average % = 87.8 | Range 84.7 - 89.6 % |
Sample type Heparin Plasma | Average % = 98.5 | Range 97 - 99.5 % |
Sample type Citrate plasma | Average % = 99 | Range 95.6 - 101 % |
Sample type Cell culture media | Average % = 107.3 | Range 103.8 - 112.9 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human MCP3 ELISA Kit (CCL7) (ab193769) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP3 (CCL7) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP3 (CCL7) with 10.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MCP3 (CCL7) is a monomeric secreted chemotactic factor of intercrine (chemokine cc) family. MCP3 attracts monocytes and eosinophils, but not neutrophils. MCP3 augments monocyte anti-tumor activity. It also induces the release of gelatinase B. MCP3 protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target MCP-3 also referred to as CCL7 is a chemotactic cytokine belonging to the CC chemokine family. Its molecular weight is approximately 11 kDa. MCP-3 is largely expressed by various cell types including monocytes lymphocytes and endothelial cells. This protein plays an important role in immune system signaling. MCP-3 exhibits chemotactic activity for monocytes T lymphocytes and natural killer (NK) cells contributing to immune cell recruitment at sites of inflammation.
- Biological function summary
MCP-3 interacts with several chemokine receptors such as CCR1 CCR2 and CCR3 allowing for diverse roles in immune response and inflammation. It functions as both a monomer and in conjunction with other chemokines to form heterodimers aiding in the modulation and fine-tuning of the immune response. The presence of MCP-3 in inflammatory tissues indicates its contribution to directing leukocyte migration and positioning therefore supporting both innate and adaptive immunity.
- Pathways
MCP-3 is a component of the chemokine signaling pathway and is significant in the regulation of immune surveillance. It interacts closely with proteins such as CCL2 and CCL3 all of which bind to similar receptors like CCR2 and CCR5. MCP-3's involvement in these pathways supports cellular responses to inflammation and infection and its activity has a direct impact on the mobilization and activation of immune cells.
- Associated diseases and disorders
MCP-3 shows associations with conditions marked by inflammation and immune system dysregulation including rheumatoid arthritis and asthma. The protein's influence in these disorders links to other chemokines like MCP-1 (CCL2) which also recruits monocytes and contributes to chronic inflammation. Disruption in MCP-3 expression or function can aggravate disease states by altering the balance of leukocyte trafficking and exacerbating inflammatory responses.
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4 product images
Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (ab193769)
Comparison of MCP3 concentrations in unstimulated and PHA-stimulated Human PBMC.
PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 48 hours. MCP3 concentrations were measured in 4 X and 8 X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and 10F RPMI1640 media. Raw data values (mean +/-SD, n=2) are graphed. The dotted line represents Blank control.
Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (ab193769)
Example of MCP3 standard curve prepared in Sample Diluent 50 BP.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (ab193769)
Titration of 48 hours PHA-stimulated PBMC cell culture supernatant samples within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (ab193769)
Example of MCP3 standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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