JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277441

Human MD1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human MD1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MD1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

MD1, LY86, Lymphocyte antigen 86, Ly-86, Protein MD-1

1 Images
ELISA - Human MD1 ELISA Kit (AB277441)
  • ELISA

Supplier Data

ELISA - Human MD1 ELISA Kit (AB277441)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.43 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human MD1 ELISA Kit (ab277441) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MD1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human MD1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MD1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MD1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MD1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "72 - 88 %", "average": "= 80.7" }, { "sample": "Plasma", "range": "76 - 88 %", "average": "= 82.62" }, { "sample": "Cell culture media", "range": "69 - 94 %", "average": "= 83.64" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277441-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277441-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277441-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277441-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human MD1 coated Microplate", "number":"AB277441-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MD1 standard protein (Lyophilized)", "number":"AB277441-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human MD1 Antibody", "number":"AB277441-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB277441-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277441-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MD1 also known as myeloid differentiation protein 1 is a small glycoprotein with a molecular mass of approximately 20 kDa. It is expressed in a variety of tissues including the spleen liver and peripheral blood leukocytes. MD1 mainly associates with the cell surface linked to its interaction with other membrane proteins. As a protein MD1 plays an important role as an accessory molecule in immune response and homeostasis.
Biological function summary

MD1 acts as an essential component of the innate immune system. It forms a heterodimeric complex with radioprotective 105 (RP105) another membrane protein. This complex is important for the modulation of Toll-like receptor 4 (TLR4) signaling pathways. By interacting with RP105 MD1 influences the recognition of pathogen-associated molecules and helps regulate inflammatory responses. This involvement is key to controlling immune cell activation.

Pathways

The TLR4 signaling pathway prominently involves MD1. This pathway is significant in detecting lipopolysaccharides (LPS) from Gram-negative bacteria. The interaction of MD1 with RP105 helps modulate this pathway adjusting the immune response to maintain balance. Additionally MD1 impacts the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway another critical route in inflammation and immune regulation. Its activity within these pathways connects it to proteins like TLR4 and MyD88.

MD1 has a notable connection with autoimmune conditions and infectious diseases. In autoimmune diseases aberrations in MD1 expression or function may lead to dysregulation of immune responses contributing to pathology. For infectious diseases like sepsis MD1’s role in the TLR4 signaling pathway can affect susceptibility and severity through its modulation of immune response. Through these pathways MD1 maintains connections with proteins such as RP105 and TLR4 highlighting its importance in disease progression and immune system dynamics.
style
left-column

Product protocols

style
left-column

Target data

May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression (By similarity).
See full target information LY86
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Proteins & Peptides

AB243755

Recombinant human Uteroglobin protein (Active)

Bioactive

  • 0
  • 0
Proteins & Peptides

AB61135

Recombinant human SHP1 protein

Functional Studies - Recombinant human SHP1 protein (AB61135)

  • 0
  • 0
Proteins & Peptides

AB243239

Recombinant mouse Prolactin/PRL protein (Active)

Bioactive

SDS-PAGE - Recombinant mouse Prolactin/PRL protein (Active) (AB243239)

  • 0
  • 0
Proteins & Peptides

AB259406

Recombinant mouse IL-4 protein (Active)

Premium Bioactive

Functional Studies - Recombinant mouse IL-4 protein (Active) (AB259406)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com