AB223866

Human MDC ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human MDC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MDC with a sensitivity of 1.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MDC, SCYA22, A-152E5.1, CCL22, C-C motif chemokine 22, CC chemokine STCP-1, MDC(1-69), Macrophage-derived chemokine, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1

6 Images

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

sELISA

Supplier Data

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Comparison of MDC in unstimulated and PHA stimulated PBMC cell supernatants.

PBMC cells were cultured for 46 hours in the presence or absence of 1.5% PHA. The concentrations of MDC were measured in three dilutions of supernatant samples in duplicates and interpolated from the MDC standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean MDC concentration was determined to be 4519 pg/mL in neat PHA stimulated PBMC cell culture supernatant and 27 pg/mL in neat unstimulated PBMC cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Interpolated concentrations of native MDC in human serum, plasma and cell culture supernatant samples.

The concentrations of MDC were measured in duplicates, interpolated from the MDC standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 45%, plasma (EDTA) 25%, and 2 days 1.5% PHA-treated PBMC supernatant 4%. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration in neat sample was determined to be 678 pg/mL in serum, 844 pg/mL in plasma (citrate), 435 pg/mL in plasma (heparin), 781 pg/ml in plasma (EDTA), and 4352 pg/ml in PHA-treated PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration was determined to be 698 pg/mL with a range of 376 – 967 pg/mL.

sELISA

Lab

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Human MDC standard curve comparison data.

Standard curve comparison between human MDC SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Serial dilutions of recombinant human 67 amino acid long MDC (aa27-93) were prepared and assayed in parallel with 69 amino acid long MDC (aa25-93), the standard of this kit.

sELISA

Supplier Data

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Example of human MDC standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human, Rhesus monkey

Assay type

Sandwich (quantitative)

Sensitivity

= 1.3 pg/mL

Range

3.1 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MDC ELISA Kit (ab223866) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MDC protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MDC with 1.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Macrophage-derived chemokine (MDC), also known as C-C motif chemokine 22 (CCL22), is a secreted cytokine and member of the intercrine beta (chemokine CC) family. It is highly expressed in macrophage and in monocyte-derived dendritic cells. MDC may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. MDC is a chemotactic for monocytes, dendritic cells and natural killer cells and potent chemoattractant for chronically activated T-lymphocytes.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 121 %", "average": "= 111" }, { "sample": "Serum", "range": "103 - 115 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "79 - 106 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "60 - 88 %", "average": "= 78" }, { "sample": "Citrate plasma", "range": "111 - 131 %", "average": "= 118" }, { "sample": "Cell culture media", "range": "98 - 102 %", "average": "= 100" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.

Biological function summary

MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.

Pathways

MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.

MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.

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Product protocols

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Target data

May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.

See full target information CCL22

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 11:1922 PubMed33042112

2020

Confirmation and Identification of Biomarkers Implicating Environmental Triggers in the Pathogenesis of Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Z Harms,Katie R Ostlund,Monina S Cabrera,Earline Edwards,Marisa Fisher,Nora Sarvetnick

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MDC ELISA Kit

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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