Human MDC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MDC in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Frontiers in immunology 11:19222020Confirmation and Identification of Biomarkers Implicating Environmental Triggers in the Pathogenesis of Type 1 Diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert Z Harms et. al.PubMed 33042112
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human, Rhesus monkey
- Range
3.1 - 200 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
Alternative names
MDC, SCYA22, A-152E5.1, CCL22, C-C motif chemokine 22, CC chemokine STCP-1, MDC(1-69), Macrophage-derived chemokine, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1
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Human MDC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MDC in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human, Rhesus monkey
- Range
3.1 - 200 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 2.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 111 | Range 102 - 121 % |
Sample type Serum | Average % = 108 | Range 103 - 115 % |
Sample type EDTA Plasma | Average % = 89 | Range 79 - 106 % |
Sample type Heparin Plasma | Average % = 78 | Range 60 - 88 % |
Sample type Citrate plasma | Average % = 118 | Range 111 - 131 % |
Sample type Cell culture media | Average % = 100 | Range 98 - 102 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human MDC ELISA Kit (ab223866) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MDC protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MDC with 1.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Macrophage-derived chemokine (MDC), also known as C-C motif chemokine 22 (CCL22), is a secreted cytokine and member of the intercrine beta (chemokine CC) family. It is highly expressed in macrophage and in monocyte-derived dendritic cells. MDC may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. MDC is a chemotactic for monocytes, dendritic cells and natural killer cells and potent chemoattractant for chronically activated T-lymphocytes.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.
- Biological function summary
MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.
- Pathways
MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.
- Associated diseases and disorders
MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.
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6 product images
Sandwich ELISA - Human MDC ELISA Kit (ab223866)
Human MDC standard curve comparison data.
Standard curve comparison between human MDC SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human MDC ELISA Kit (ab223866)
Example of human MDC standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MDC ELISA Kit (ab223866)
Interpolated concentrations of native MDC in human serum, plasma and cell culture supernatant samples.
The concentrations of MDC were measured in duplicates, interpolated from the MDC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 45%, plasma (EDTA) 25%, and 2 days 1.5% PHA-treated PBMC supernatant 4%. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration in neat sample was determined to be 678 pg/mL in serum, 844 pg/mL in plasma (citrate), 435 pg/mL in plasma (heparin), 781 pg/ml in plasma (EDTA), and 4352 pg/ml in PHA-treated PBMC supernatant.
Sandwich ELISA - Human MDC ELISA Kit (ab223866)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration was determined to be 698 pg/mL with a range of 376 – 967 pg/mL.
Sandwich ELISA - Human MDC ELISA Kit (ab223866)
Comparison of MDC in unstimulated and PHA stimulated PBMC cell supernatants.
PBMC cells were cultured for 46 hours in the presence or absence of 1.5% PHA. The concentrations of MDC were measured in three dilutions of supernatant samples in duplicates and interpolated from the MDC standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean MDC concentration was determined to be 4519 pg/mL in neat PHA stimulated PBMC cell culture supernatant and 27 pg/mL in neat unstimulated PBMC cell culture supernatant.
Sandwich ELISA - Human MDC ELISA Kit (ab223866)
Serial dilutions of recombinant human 67 amino acid long MDC (aa27-93) were prepared and assayed in parallel with 69 amino acid long MDC (aa25-93), the standard of this kit.
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