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AB255724

Human MDH1 ELISA Kit (cytosolic malate dehydrogenase)

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Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) is a single-wash 90-min Simplestep used to quantify Human MDH1 (cytosolic malate dehydrogenase) with a sensitivity of 64 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MDHA, MDH1, Aromatic alpha-keto acid reductase, Cytosolic malate dehydrogenase, KAR

2 Images
Sandwich ELISA - Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (AB255724)
  • sELISA

Supplier Data

Sandwich ELISA - Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (AB255724)

Interpolated concentrations of native MDH1 in human serum and plasma samples.

The concentrations of MDH1 were measured in duplicates, interpolated from the MDH1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 12.5% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MDH1 concentration was determined to be 15.35 ng/mL in serum, 21.69 ng/mL in plasma (citrate), 15.07 ng/mL in plasma (heparin) and 13.22 ng/mL in plasma (EDTA).

Sandwich ELISA - Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (AB255724)
  • sELISA

Supplier Data

Sandwich ELISA - Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (AB255724)

Example of human MDH1 standard curve in 1X Cell Extraction Buffer PTR.

The MDH1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 64 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MDH1 ELISA Kit (cytosolic malate dehydrogenase) (ab255724) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MDH1 (cytosolic malate dehydrogenase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MDH1 (cytosolic malate dehydrogenase) with 64 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MDH1 is the cytosolic form of malate dehydrogenase, an enzyme that catalyzes the oxidation of malate to oxaloacetate while concomitantly reducing NAD+ to NADH. Its classical role is the malate:aspartate shuttle, where it facilitates the transfer of electrons produced during glycolysis into the mitochondria to produce ATP. Beyond its classical role, this enzyme may play a primary regulatory role in adipogenesis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 101 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "88 - 103 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "94 - 103 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "85 - 101 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cytosolic malate dehydrogenase commonly called MDH1 or malate dehydrogenase 1 is an enzyme that plays an important role in the citric acid cycle. Its main function is to catalyze the reversible conversion of malate to oxaloacetate using NAD+ as a cofactor. This enzyme has an approximate molecular mass of 36 kDa. MDH1 is expressed in the cytosol of various cell types throughout the body. It is critical in enabling cells to efficiently convert biochemical energy from nutrients into ATP which is vital for cellular function and survival.
Biological function summary

This enzyme supports numerous cellular processes by maintaining redox balance and facilitating energy production. MDH1 acts independently and isn't part of any larger protein complex. However its efficiency directly impacts the cycling of metabolites necessary for cellular respiration and energy metabolism. By converting malate into oxaloacetate MDH1 directly influences processes critical for the maintenance of cell health and function.

Pathways

MDH1 is an integral component of the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle it plays an important role in the conversion steps that drive energy generation within cells. Additionally the malate-aspartate shuttle is an important pathway connecting cytosolic and mitochondrial processes by facilitating the exchange of reducing equivalents. Through these pathways MDH1 interacts and collaborates with proteins like aspartate aminotransferase (GOT1) and mitochondrial malate dehydrogenase (MDH2) showcasing the interconnected nature of metabolic pathways.

Dysfunction or abnormal expression of MDH1 has been associated with various conditions including cancer and metabolic syndromes. In cancer aberrant MDH1 activity can lead to altered energy metabolism promoting tumor progression and survival. Additionally MDH1's role in glucose and lipid metabolism makes it relevant in metabolic syndrome where improper energy balance can exacerbate disease states. Proteins connected through these associations include hypoxia-inducible factor 1-alpha (HIF-1α) in cancer metabolism and insulin signaling proteins in metabolic syndromes further illustrating the broader implications of MDH1's function in health and disease.
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Product protocols

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Target data

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed : 2449162, PubMed : 3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed : 31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed : 34012073).
See full target information MDH1
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websiteProtocolBooklet
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Product promise

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