Human MEF2A ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MEF2A in Tissue Extracts, Cell Lysate samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.188 - 12 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.055 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.
Alternative names
MEF2, MEF2A, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1
What's included?
Recommended products
Human MEF2A ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MEF2A in Tissue Extracts, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.188 - 12 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.055 ng/mL
- General recovery
- 93%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 5.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 4.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 93 | Range 81 - 107 % |
Sample type Tissue Extracts | Average % = 112 | Range 106 - 123 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human MEF2A SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MEF2A protein in Cell Lysate, Tissue extracts . Quantitate Human MEF2A with 0.055 ng/ml sensitivity.SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEF2A or Myocyte Enhancer Factor 2A is a transcription factor involved in regulating gene expression. MEF2A is approximately 55 kDa in size and is expressed in a variety of tissues including muscle brain and heart. It is part of the MEF2 family of transcription factors which all share a similar MADS-box/MEF2 domain structure. MEF2A binds to DNA and influences the transcription of genes involved in muscle differentiation and development.
- Biological function summary
MEF2A participates in transcriptional regulation as part of a larger complex that includes other regulatory proteins. This transcription factor plays an essential role in the development and function of muscles. It activates the transcription of genes necessary for muscle fiber formation and adaptation. MEF2A also influences the expression of genes related to neuronal survival and plasticity impacting cognitive function and memory in the brain.
- Pathways
MEF2A is involved in both the MAPK/ERK and PI3K/Akt pathways which are significant for cellular growth survival and differentiation. MEF2A interacts with proteins like MAPK and PI3K impacting these signaling pathways that regulate cell proliferation and apoptosis. This interaction highlights its role in cellular response to external stimuli and stress signals positioning MEF2A as an important factor in cell fate decisions.
- Associated diseases and disorders
Mutations or dysregulation of MEF2A are connected to cardiovascular diseases particularly coronary artery disease. MEF2A mutations can disrupt normal transcriptional regulation leading to impaired cardiovascular function. Additionally alterations in MEF2A function are linked to neurodegenerative disorders such as Alzheimer's disease where it may interact with proteins including Tau and amyloid precursor protein influencing neuronal health and survival.
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2 product images
Sandwich ELISA - Human MEF2A ELISA Kit (ab314602)
Example of human MEF2A standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MEF2A ELISA Kit (ab314602)
Interpolated concentration of native MEF2A was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HeLa cell extract 125 µg/mL, and heart tissue extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Product protocols
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