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AB315315

Human MEF2B ELISA Kit

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Human MEF2B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MEF2B with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

XMEF2, MEF2B, Myocyte-specific enhancer factor 2B, RSRFR2, Serum response factor-like protein 2

2 Images
Sandwich ELISA - Human MEF2B ELISA Kit (AB315315)
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Supplier Data

Sandwich ELISA - Human MEF2B ELISA Kit (AB315315)

Interpolated concentrations of human MEF2B in Ramos cell extract

Interpolated concentration of native MEF2B was measured in duplicate at different sample concentrations. Undiluted samples are 200 µg/mL Ramos cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human MEF2B ELISA Kit (AB315315)
  • sELISA

Supplier Data

Sandwich ELISA - Human MEF2B ELISA Kit (AB315315)

Example of human MEF2B standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.7 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB242058

Anti-MEF2B antibody [EPR22193-26]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MEF2B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MEF2B protein in Cell Lysate. Quantitate Human MEF2B with 4.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.9" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "108 - 114 %", "average": "= 111" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEF2B also known as myocyte enhancer factor 2B plays an integral role in regulating gene expression. It is a transcription factor belonging to the MEF2 family which also includes MEF2A MEF2C and MEF2D. MEF2B has a molecular mass of approximately 50 kilodaltons. Researchers have found its expression mainly in lymphoid tissues but it is also present in other tissue types. Through its actions MEF2B influences various cellular processes by binding to DNA and facilitating transcriptional activation.
Biological function summary

MEF2B functions as a regulator of immune system development and differentiation. It acts at the level of transcription to determine the fate of cells by its interactions. MEF2B often partners with various cofactors to form a complex which enhances its ability to control gene expression. It is known to be highly involved in muscle development and cell proliferation underlining its diverse roles in cellular biology.

Pathways

MEF2B plays important roles in two key signaling networks: the MAPK signaling pathway and the calcium signaling pathway. These pathways manage diverse biological processes like cell growth apoptosis and differentiation. Through the MAPK pathway MEF2B interacts with proteins such as ERK leading to various cellular outcomes. In the calcium signaling pathway MEF2B works alongside calcineurin a phosphatase important for activating MEF2 family members.

MEF2B has been linked with specific health conditions particularly in lymphoma and leukemia. Mutations and dysregulation in MEF2B may contribute to the development of these cancers. MEF2B's interaction with other proteins like BCL6 a well-known repressor in lymphomagenesis highlights its importance in these contexts. Understanding MEF2B's role in these diseases may provide insights for potential therapeutic strategies.
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Product protocols

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Target data

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.
See full target information MEF2B
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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