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Human Melan-A ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Melan-A in Human in Serum, Plasma, Cell culture supernatant samples.

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Images

Sandwich ELISA - Human Melan-A ELISA Kit (AB309421), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.085 - 10 ng/mL
Sensitivity
= 0.045 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
800X HRP-Streptavidin
1 x 200 µL
Biotinylated Human Melan-A/MART-1 Detection Antibody
2 x 1 Vial
Human Melan-A/MART-1 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human Melan-A/MART-1 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human Melan-A ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Melan-A in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.085 - 10 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.045 ng/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 114.5
Range
72 - 140 %
Sample type
Plasma
Average %
= 97.79
Range
68 - 141 %
Sample type
Cell culture media
Average %
= 102.4
Range
70 - 135 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human MelanA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MelanA in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human MelanA coated on a 96-well plate.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human Melan-A ELISA Kit (ab309421), expandable thumbnail

    Sandwich ELISA - Human Melan-A ELISA Kit (ab309421)

    Example of standard curve using ab309421. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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