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AB309421

Human Melan-A ELISA Kit

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Human Melan-A ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Melan-A in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

MART1, MLANA, Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A

1 Images
Sandwich ELISA - Human Melan-A ELISA Kit (AB309421)
  • sELISA

Supplier Data

Sandwich ELISA - Human Melan-A ELISA Kit (AB309421)

Example of standard curve using ab309421. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.045 ng/mL

Range

0.085 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human MelanA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MelanA in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human MelanA coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "72 - 140 %", "average": "= 114.5" }, { "sample": "Plasma", "range": "68 - 141 %", "average": "= 97.79" }, { "sample": "Cell culture media", "range": "70 - 135 %", "average": "= 102.4" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309421-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309421-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Melan-A/MART-1 Protein Standard", "number":"AB309421-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309421-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309421-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Melan-A/MART-1 Detection Antibody", "number":"AB309421-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309421-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin", "number":"AB309421-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Melan-A/MART-1 Antibody-coated ELISA Plate", "number":"AB309421-CMP08", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
See full target information MLANA
websiteProtocolBooklet
en

Product promise

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