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AB277405

Human MEPE ELISA Kit

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Human MEPE ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MEPE in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Matrix extracellular phosphoglycoprotein, Osteoblast/osteocyte factor 45, Osteoregulin, OF45, MEPE

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ELISA - Human MEPE ELISA Kit (AB277405)
  • ELISA

Supplier Data

ELISA - Human MEPE ELISA Kit (AB277405)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.4 ng/mL

Range

8.192 - 8000 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MEPE ELISA Kit (ab277405) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MEPE in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant; Heparin is not recommended for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Human MEPE coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MEPE present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MEPE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MEPE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "69 - 95 %", "average": "= 80.13" }, { "sample": "Serum", "range": "69 - 103 %", "average": "= 87.11" }, { "sample": "Plasma", "range": "93 - 105 %", "average": "= 98.58" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277405-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277405-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB277405-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277405-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human MEPE coated Microplate", "number":"AB277405-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277405-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277405-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MEPE standard protein (Lyophilized)", "number":"AB277405-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human MEPE Antibody", "number":"AB277405-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEPE also known as Matrix Extracellular Phosphoglycoprotein is a protein involved in bone metabolism. It has a molecular mass of approximately 56 kDa. MEPE is expressed in osteoblasts and osteocytes cells critical for bone formation and maintenance. This protein also exists in other tissues like kidney and dental pulp where it could possibly have additional roles.
Biological function summary

MEPE contributes to the regulation of bone mineralization and phosphate homeostasis. The protein functions as part of a complex mechanism that modulates bone density responding to signals for bone growth or resorption. Its role involves binding to hydroxyapatite a mineral component of bone which suggests MEPE importantly influences bone matrix structure and strength.

Pathways

MEPE participates in the regulation of the phosphate and vitamin D pathways. It interacts closely with proteins such as PHEX (Phosphate-regulating gene with Homologies to Endopeptidases on the X chromosome) in these pathways. MEPE’s involvement in phosphate regulation affects both renal phosphate handling and intestinal absorption of dietary phosphate hinting at its role in broader systemic calcium and phosphate balance.

MEPE shows connections with disorders like X-linked hypophosphatemic rickets and tumor-induced osteomalacia. These conditions involve abnormal phosphate metabolism and bone degradation. The dysfunction of MEPE along with proteins like PHEX may result in impaired bone mineralization leading to clinical symptoms associated with these conditions such as bone pain and skeletal deformities.
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Product protocols

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Target data

Promotes renal phosphate excretion and inhibits intestinal phosphate absorption (PubMed : 14962809, PubMed : 19005008). Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed : 18162525, PubMed : 19998030, PubMed : 22766095). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (By similarity). Promotes dental pulp stem cell proliferation and differentiation (PubMed : 22341070).
See full target information MEPE
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