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AB119604

Human MER ELISA Kit

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(3 Publications)

Human MER ELISA Kit is a Sandwich ELISA for the measurement of Human MER in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Mer, Mertk, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

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Sandwich ELISA - Human MER ELISA Kit (AB119604)
  • sELISA

Supplier Data

Sandwich ELISA - Human MER ELISA Kit (AB119604)

Representative standard curve using ab119604

Key facts

Detection method

Colorimetric

Sample types

Tissue Homogenate, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human MER in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MER in cell culture supernatants, cell lysates, tissue homogenates and serum.

A MER specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for MER is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MER amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "217 pg/mL", "standardDeviation": "13.67", "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1701 pg/mL", "standardDeviation": "105.46", "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4920 pg/mL", "standardDeviation": "221.4", "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "236 pg/mL", "standardDeviation": "14.63", "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1665 pg/mL", "standardDeviation": "79.92", "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4937 pg/mL", "standardDeviation": "197.48", "coefficientOfVariability": "4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119604-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119604-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human MER antibody", "number":"AB119604-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human MER antibody Microplate (12 x 8 wells)", "number":"AB119604-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119604-CMP06", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119604-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119604-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119604-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119604-CMP07", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant Human MER standard", "number":"AB119604-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119604-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119604-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.
Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.
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Product protocols

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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
See full target information Mertk
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Environmental pollution (Barking, Essex : 1987) 355:124151 PubMed38740242

2024

Elevated plasma solMER concentrations link ambient PM and PAHs to myocardial injury and reduced left ventricular systolic function in children.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Chen,Xia Huo,Yu Huang,Zhiheng Cheng,Xijin Xu,Zhi Li

Journal of obesity 2024:1424404 PubMed38550672

2024

The Important Role of Phosphatidylserine, ADAM17, TNF-Alpha, and Soluble MER on Efferocytosis Activity in Central Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Chandra Agung Purnama,Anna Meiliana,Melisa Intan Barliana,Keri Lestari,Andi Wijaya

Cell reports 28:172-189.e7 PubMed31269438

2019

The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhou Hou,Guobao Chen,William Bracamonte-Baran,Hee Sun Choi,Nicola L Diny,Jungeun Sung,David Hughes,Taejoon Won,Megan Kay Wood,Monica V Talor,David Joel Hackam,Karin Klingel,Giovanni Davogustto,Heinrich Taegtmeyer,Isabelle Coppens,Jobert G Barin,Daniela Čiháková
View all publications
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Product promise

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