Human Met (c-Met) ELISA Kit
- Recombinant
- SimpleStep
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Human Met (c-Met) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Met (c-Met) with a sensitivity of 81.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, MET
- sELISA
Supplier Data
Sandwich ELISA - Human Met (c-Met) ELISA Kit (AB277722)
Extraction Buffer PTR.
The Met standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Met (c-Met) ELISA Kit (AB277722)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Met concentration was determined to be 88.5 ng/mL with a range of 71.3 – 114.2 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Met (c-Met) ELISA Kit (AB277722)
Interpolated concentrations of native Met in human A431 cell based on a 250 μg/mL extract load.
The concentrations of Met were measured in duplicate and interpolated from the Met standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Met concentration was determined to be 12.8 ng/mL in A431 extract.
- sELISA
Supplier Data
Sandwich ELISA - Human Met (c-Met) ELISA Kit (AB277722)
Example of human Met standard curve in Sample Diluent NS.
The Met standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Met (c-Met) ELISA Kit (AB277722)
Interpolated concentrations of native Met in human serum and plasma samples.
The concentrations of Met were measured in duplicates, interpolated from the Met standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Met concentration was determined to be 111.4 ng/mL in neat serum, 90.7 ng/mL in neat plasma (citrate), 94.1 ng/mL in neat plasma (EDTA) and 89.0 ng/mL in neat plasma (heparin).
Reactivity data
Product details
Human Met (c-Met) ELISA kit (ab277722) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Met (c-Met) protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Met (c-Met) with 81.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Met, also known as Hepatocyte Growth Factor Receptor (HGFR)or Proto-Oncogene c-Met (c-Met) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor ligand. Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of Met on its intracellular domain that provides docking sites for downstream signaling molecules. The recruitment of these downstream effectors by Met leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLC gamma-PKC. During embryonic development, Met signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, Met participates in wound healing as well as organ regeneration and tissue remodeling. Met promotes also differentiation and proliferation of hematopoietic cells. Human and mouse Met are 90% identical on their amino acids sequences.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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