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AB309279

Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit

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Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Metabotropic Glutamate Receptor 3/MGLUR3 with a sensitivity of 9.14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GPRC1C, MGLUR3, GRM3, Metabotropic glutamate receptor 3, mGluR3

2 Images
Sandwich ELISA - Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit (AB309279)
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Supplier Data

Sandwich ELISA - Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit (AB309279)

Example of human Metabotropic Glutamate Receptor 3/MGLUR3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit (AB309279)
  • sELISA

Supplier Data

Sandwich ELISA - Human Metabotropic Glutamate Receptor 3/MGLUR3 ELISA Kit (AB309279)

Interpolated concentration of native Metabotropic Glutamate Receptor 3/MGLUR3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human hippocampus extract 200 μg/mL, mouse brain extract 100 μg/mL, and rat brain extract 200 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9.14 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB166608

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)]

RabMAb|Recombinant

Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (AB166608)

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Biochemicals

AB120002

L-AP4, group III mGlu agonist

Chemical Structure - L-AP4, group III mGlu agonist (AB120002)

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Cell Lines & Lysates

AB306690

Human GRM3 knockout U-87 MG cell line

Next Generation Sequencing - Human GRM3 knockout U-87 MG cell line (AB306690)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Metabotropic Glutamate Receptor 3/MGLUR3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Metabotropic Glutamate Receptor 3/MGLUR3 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Metabotropic Glutamate Receptor 3/MGLUR3 with 9.14 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "97 - 113 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309279-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309279-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309279-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309279-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309279-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309279-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB309279-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Metabotropic Glutamate Receptor 3/MGLUR3 Lyophilized Recombinant Protein", "number":"AB309279-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Metabotropic Glutamate Receptor 3/MGLUR3 Capture Antibody", "number":"AB309279-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309279-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB309279-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Metabotropic Glutamate Receptor 3/MGLUR3 Detector Antibody", "number":"AB309279-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
See full target information GRM3
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com