Human Methionine Aminopeptidase 2/p67 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Methionine Aminopeptidase 2/p67 in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2 - 250 ng/mL
- Sensitivity
- = 1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
Alternative names
MNPEP, P67EIF2, METAP2, Methionine aminopeptidase 2, MAP 2, MetAP 2, Initiation factor 2-associated 67 kDa glycoprotein, Peptidase M, p67, p67eIF2
What's included?
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Human Methionine Aminopeptidase 2/p67 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Methionine Aminopeptidase 2/p67 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2 - 250 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 118.2 | Range 114 - 124 % |
Sample type Plasma | Average % = 117.7 | Range 95 - 139 % |
Sample type Cell culture media | Average % = 77.63 | Range 76 - 81 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Methionine Aminopeptidase 2/p67 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Methionine Aminopeptidase 2/p67 in serum (human Methionine Aminopeptidase 2/p67 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants. This assay employs an antibody specific for human Methionine Aminopeptidase 2/p67 coated on a 96-well plate.
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1 product image
Sandwich ELISA - Human Methionine Aminopeptidase 2/p67 ELISA Kit (ab313992)
Example of standard curve using ab313992. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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