AB313992
Human Methionine Aminopeptidase 2/p67 ELISA Kit
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Human Methionine Aminopeptidase 2/p67 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Methionine Aminopeptidase 2/p67 in Human in Biofluids, Cell Culture Media samples.
View Alternative Names
MNPEP, P67EIF2, METAP2, Methionine aminopeptidase 2, MAP 2, MetAP 2, Initiation factor 2-associated 67 kDa glycoprotein, Peptidase M, p67, p67eIF2
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Methionine Aminopeptidase 2/p67 ELISA Kit (AB313992)
Example of standard curve using ab313992. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human Methionine Aminopeptidase 2/p67 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Methionine Aminopeptidase 2/p67 in serum (human Methionine Aminopeptidase 2/p67 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants. This assay employs an antibody specific for human Methionine Aminopeptidase 2/p67 coated on a 96-well plate.
Recovery
[ { "sample": "Serum", "range": "114 - 124 %", "average": "= 118.2" }, { "sample": "Plasma", "range": "95 - 139 %", "average": "= 117.7" }, { "sample": "Cell culture media", "range": "76 - 81 %", "average": "= 77.63" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
The protein expressed by the MAP2 gene cotranslationally removes the N-terminal methionine from nascent proteins, particularly when the second residue is small and uncharged. In comparison to METAP1, the catalytic activity of human METAP2 towards Met-Val peptides is consistently much higher, indicating its role in processing proteins with N-terminal Met-Val and Met-Thr sequences in vivo. Additionally, it protects the eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases, such as EIF2AK2/PKR and EIF2AK1/HCR, playing a critical role in the regulation of protein synthesis. This supplementary information is collated from multiple sources and compiled automatically.
Complementary Products
Primary Antibodies
AB134124
Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887]
RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (AB134124)](https://content.abcam.com/products/images/methionine-aminopeptidase-2-p67-antibody-epr6887-ab134124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133648.jpg)
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