AB235638

Human MFGE8 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human MFGE8 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MFGE8 with a sensitivity of 2.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, SED1, MFG-E8, MFGE8

9 Images

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Interpolated concentrations of native MFGE8 in human milk and A549 cell culture supernatant samples.

The concentrations of MFGE8 were measured in duplicates, interpolated from the MFGE8 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 20,000 and A549 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 6794 ng/mL in neat milk and 7.260 ng/mL in neat A549 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Example of human MFGE8 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 260.5 pg/mL with a range of 98.60 – 406.6 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Human MFGE8 Competitor Std Curve Comparison.

Standard curve comparison between human MFGE8 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Interpolated concentrations of spike and native MFGE8 in human serum and plasma (citrate).

The concentrations of MFGE8 were measured in duplicates, interpolated from the MFGE8 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25% and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean native MFGE8 concentration was determined to be 694.7 pg/mL in neat serum and 178.1 pg/mL in neat plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Interpolated concentrations of native MFGE8 in human A549 cell extract, human liver tissue extract and human placenta tissue extract.

Interpolated concentrations of native MFGE8 in human A549 cell extract based on a 25 μg/mL extract load, human liver tissue extract based on a 400 μg/mL extract load, and human placenta tissue extract based on a 200 μg/mL extract load. The concentrations of MFGE8 were measured in duplicate and interpolated from the MFGE8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 756.6 pg/mL in A549 cell extract, 230.1 pg/mL in human liver tissue extract, and 658.5 pg/mL in human placenta tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Comparison of native human MFGE8 in cell and tissue extracts expressed in ng of MFGE8 per mg of extract.

The concentrations of MFGE8 were measured in duplicate and interpolated from the MFGE8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 29.24 ng/mg in A549 cell extract, 0.546 ng/mg in human liver tissue extract, and 3.222 ng/mg in human placenta tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Example of human MFGE8 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Example of human MFGE8 standard curve in Sample Diluent 10BS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Citrate plasma, Cell culture supernatant, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.4 pg/mL

Range

15.6 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MFGE8 ELISA Kit (ab235638) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MFGE8 protein in cell culture supernatant, cit plasma, milk, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MFGE8 with 2.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 114 %", "average": "= 103" }, { "sample": "Milk", "range": "80 - 91 %", "average": "= 86" }, { "sample": "Serum", "range": "121 - 128 %", "average": "= 124" }, { "sample": "Cell culture extracts", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Tissue Extracts", "range": "88 - 98 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "117 - 124 %", "average": "= 121" }, { "sample": "Cell culture media", "range": "88 - 98 %", "average": "= 94" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.. Medin. Main constituent of aortic medial amyloid.

See full target information MFGE8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2022:6731286 PubMed36267465

2022

Elevated MFG-E8 in CSF in the Early Stage Indicates Rapid Recovery of Mild Aneurysmal SAH Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Pang,Zheng Peng,Xiaojian Li,Yongyue Gao,Xunzhi Liu,Han Wang,Yue Lu,Zong Zhuang,Qingrong Zhang,Wei Li,Chunhua Hang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MFGE8 ELISA Kit

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Sandwich ELISA - Human MFGE8 ELISA Kit (AB235638)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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