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AB284030

Human MGMT ELISA Kit

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Human MGMT ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MGMT in Human in Cell/Tissue Extracts samples.

View Alternative Names

Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, MGMT

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Sandwich ELISA - Human MGMT ELISA Kit (AB284030)
  • sELISA

Supplier Data

Sandwich ELISA - Human MGMT ELISA Kit (AB284030)

Example of Human MGMT standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.5 ng/mL

Range

7.813 - 125 ng/mL

Assay time

5h

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human MGMT ELISA Kit (ab284030) is designed for detection of MGMT in human cell lysate samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human MGMT in approximately 5 hours. A polyclonal antibody specific for human MGMT has been pre-coated onto a 96-well microplate with removable strips. MGMT in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human MGMT, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGMT also known as O-6-methylguanine-DNA methyltransferase is an enzyme with a significant role in DNA repair. MGMT protein has a molecular weight of about 22 kDa. This protein is typically expressed in various tissues including the liver kidney and brain where it functions to protect cells from mutagenic and cytotoxic alterations. MGMT works by transferring alkyl groups from the O6 position of guanine to a cysteine residue within its active site. This mechanism directly reverses DNA damage maintaining genomic stability.
Biological function summary

MGMT prevents mutations by reversing alkylation damage playing a significant role in preserving DNA integrity. It acts independently and does not form part of any larger protein complexes. This enzyme-mediated repair is central to cellular defense against mutagenic threats such as alkylating agents. By curbing DNA damage MGMT can thwart the onset of harmful genetic mutations that might lead to issues like cancer development.

Pathways

MGMT is intimately involved in DNA damage response and repair pathways. Notably it interacts with base excision repair and direct reversal repair pathways. MGMT's function is distinct but complementary to the activities of other DNA repair proteins like XRCC1 and APE1 which are involved in the base excision repair pathway. The presence of MGMT bolsters cellular resilience by ensuring that DNA lesions caused by alkylating agents are addressed promptly and effectively.

MGMT has a profound association with several types of cancer such as glioblastoma and colorectal cancer. Reduced MGMT expression or activity can lead to enhanced susceptibility to the carcinogenic effects of alkylating agents making cancer development more likely. Additionally MGMT inhibitors such as lomeguatrib are used therapeutically to enhance the effectiveness of alkylating chemotherapy. Within this context MGMT interacts with proteins involved in tumor suppression and cell cycle regulation including p53 influencing both cancer progression and treatment outcomes.
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Product protocols

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Target data

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction : the enzyme is irreversibly inactivated.
See full target information MGMT
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