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AB171340

Human MIA ELISA Kit

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Human MIA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MIA in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Melanoma-derived growth regulatory protein, Melanoma inhibitory activity protein, MIA

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Sandwich ELISA - Human MIA ELISA Kit (AB171340)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIA ELISA Kit (AB171340)

Representative standard curve using ab171340

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

15.6 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human MIA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MIA in cell culture supernatants, serum and plasma (heparin, EDTA) and saliva.

A MIA specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MIA is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MIA amount of sample captured in plate.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171340-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171340-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human MIA antibody", "number":"AB171340-CMP09", "productcode":"" }, { "size":"1 x 96 Test", "name":"Anti-Human MIA Antibody Microplate (12 x 8 wells)", "number":"AB171340-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171340-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171340-CMP10", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171340-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171340-CMP08", "productcode":"" }, { "size":"2 x 1 ng", "name":"Lyophilized recombinant Human MIA standard", "number":"AB171340-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171340-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171340-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171340-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
See full target information MIA
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com