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AB100592

Human MICA ELISA Kit

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(5 Publications)

Human MICA ELISA Kit is a Sandwich ELISA for the measurement of Human MICA in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A

3 Images
ELISA - Human MICA ELISA Kit (AB100592)
  • ELISA

Lab

ELISA - Human MICA ELISA Kit (AB100592)

Standard curve with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Human MICA ELISA Kit (AB100592)
  • sELISA

Supplier Data

Sandwich ELISA - Human MICA ELISA Kit (AB100592)

Representative Standard Curve using ab100592.

ELISA - Human MICA ELISA Kit (AB100592)
  • ELISA

Lab

ELISA - Human MICA ELISA Kit (AB100592)

MICA measured in undiluted cell culture supernatants, U937 and THP-1 signals were below level of detection (13.7 pg x mL-1) (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 20 pg/mL

Range

13.72 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MICA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MICA in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human MICA coated on a 96-well plate. Standards and samples are pipetted into the wells and MICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "109 - 124 %", "average": "= 116.9" }, { "sample": "Serum", "range": "95 - 129 %", "average": "= 112.3" }, { "sample": "Plasma", "range": "75 - 107 %", "average": "= 93.83" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100592-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100592-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100592-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human MICA", "number":"AB100592-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MICA Microplate (12 x 8 wells)", "number":"AB100592-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human MICA Standard (lyophilized)", "number":"AB100592-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100592-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100592-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100592-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICA also known as MHC class I polypeptide-related sequence A is a protein encoded by the MICA gene. It has a molecular weight of approximately 62 kDa. MICA is a stress-induced ligand expressed on the surface of various cell types including epithelial cells fibroblasts and some tumor cells. This protein is part of the MHC class I chain-related (MIC) family which plays an important role in the immune system by interacting with natural killer (NK) cells and certain T cells.
Biological function summary

The MICA protein acts as a ligand for the NKG2D receptor an activating receptor found on NK cells and some T-cell subsets. MICA does not require expression as part of a complex with β2-microglobulin for function. When cells express MICA they signal distress allowing immune cells to target and destroy potentially harmful cells such as those infected with viruses or transformed into cancer cells. This interaction is an important component of the immune surveillance system.

Pathways

MICA functions within the natural cytotoxicity effector pathway heavily involved in immune responses. It is also relevant in the DNA damage response pathways. Within these pathways MICA interacts with proteins like NKG2D and plays a part in mediating cellular responses to stress and damage. These interactions enhance the capacity of immune cells to recognize and eliminate abnormal cells.

MICA is associated with conditions such as psoriasis and certain types of cancer. Studies have shown increased levels of soluble MICA in the sera of patients with psoriasis indicating a role in inflammation. Additionally in cancer MICA expression affects tumor immunity connecting to the NKG2D receptor to influence immune cell targeting and destruction of tumor cells. Researchers use tools such as MICA ELISA kits and imaging techniques to study its role in these diseases.
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Product protocols

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Target data

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).
See full target information MICA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:307 PubMed39048814

2024

CircARAP2 controls sMICA-induced NK cell desensitization by erasing CTCF/PRC2-induced suppression in early endosome marker RAB5A.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Guo,Nawen Du,Xue Wen,Zhaozhi Li,Yantong Guo,Lei Zhou,Andrew R Hoffman,Lingyu Li,Ji-Fan Hu,Jiuwei Cui

Nature 606:992-998 PubMed35614223

2022

A vaccine targeting resistant tumours by dual T cell plus NK cell attack.

Applications

Unspecified application

Species

Unspecified reactive species

Soumya Badrinath,Maxence O Dellacherie,Aileen Li,Shiwei Zheng,Xixi Zhang,Miguel Sobral,Jason W Pyrdol,Kathryn L Smith,Yuheng Lu,Sabrina Haag,Hamza Ijaz,Fawn Connor-Stroud,Tsuneyasu Kaisho,Glenn Dranoff,Guo-Cheng Yuan,David J Mooney,Kai W Wucherpfennig

Oncoimmunology 11:2035919 PubMed35223192

2022

MICA/B-targeted antibody promotes NK cell-driven tumor immunity in patients with intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Oliviero,Stefania Varchetta,Dalila Mele,Greta Pessino,Roberta Maiello,Monica Falleni,Delfina Tosi,Matteo Donadon,Cristiana Soldani,Barbara Franceschini,Guido Torzilli,Gaetano Piccolo,Matteo Barabino,Enrico Opocher,Marcello Maestri,Stefano Bernuzzi,Kai W Wucherpfennig,Mario U Mondelli,Stefania Mantovani

Oncoimmunology 5:e1048061 PubMed26942056

2016

Failure recovery of circulating NKG2DCD56NK cells in HBV-associated hepatocellular carcinoma after hepatectomy predicts early recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Gao,Zhaojun Duan,Ling Zhang,Xiangbo Huang,Lu Long,Jing Tu,Hua Liang,Yu Zhang,Tao Shen,Fengmin Lu

Cellular & molecular immunology 12:768-76 PubMed25363527

2014

Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ren,Yunzhong Nie,Mingming Lv,Sunan Shen,Ruijing Tang,Yujun Xu,Yayi Hou,Shuli Zhao,Tingting Wang
View all publications
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Product promise

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