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AB100593

Human MICB ELISA Kit

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(3 Publications)

Human MICB ELISA Kit is a Sandwich ELISA for the measurement of Human MICB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PERB11.2, MICB, MHC class I polypeptide-related sequence B, MIC-B

2 Images
Sandwich ELISA - Human MICB ELISA Kit (AB100593)
  • sELISA

Supplier Data

Sandwich ELISA - Human MICB ELISA Kit (AB100593)

Representative Standard Curve using ab100593.

Sandwich ELISA - Human MICB ELISA Kit (AB100593)
  • sELISA

Supplier Data

Sandwich ELISA - Human MICB ELISA Kit (AB100593)

Representative Standard Curve using ab100593.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 75 pg/mL

Range

0.0686 - 50 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MICB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MICB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human MICB coated on a 96-well plate. Standards and samples are pipetted into the wells and MICB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 125 %", "average": "= 98.22" }, { "sample": "Serum", "range": "102 - 122 %", "average": "= 113.1" }, { "sample": "Plasma", "range": "65 - 88 %", "average": "= 75.47" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100593-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100593-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100593-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100593-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MICB Microplate (12 x 8 wells)", "number":"AB100593-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100593-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100593-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human MICB", "number":"AB100593-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human MICB Standard (lyophilized)", "number":"AB100593-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100593-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICB also known as MHC class I polypeptide-related sequence B is a protein with a molecular weight of around 42 kDa. It anchors on cell surfaces and is widely expressed in epithelial cells fibroblasts and monocytes. MICB surfaces on stressed cells but remains minimal in normal conditions indicating its involvement in cell stress response. It is an activating ligand for NKG2D receptor on natural killer (NK) cells and certain T cells playing a major role in immune surveillance.
Biological function summary

MICB contributes to immune system functioning by presenting stress-induced antigens to immune cells. It does not belong to a complex but engages in interactions critical for activating cytotoxic responses. By binding to the NKG2D receptor it helps activate NK cells and certain T cell subsets which results in the elimination of damaged or transformed cells. This immune activation is important for the surveillance against cancer and infected cells maintaining tissue homeostasis.

Pathways

MICB plays a role in the stress-induced NKG2D signaling pathway and is involved in the immune response pathway. It cooperates with proteins such as NKG2D and MICA another ligand of the NKG2D receptor to initiate immune cell-mediated cytotoxicity. These pathways activate NK cells and CD8+ T cells important for removing stressed or altered cells thereby influencing the body's defense mechanisms.

MICB has connections to cancer and autoimmune disorders. Aberrant expression of MICB appears in tumors affecting tumor immune evasion and associates with autoimmune diseases influencing immune tolerance. Dysregulation of MICB activity can affect interactions with proteins like NKG2D altering immune responses and contributing to disease progression. Therefore understanding MICB's role is critical for developing therapeutic interventions targeting immune-related diseases.
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Product protocols

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Target data

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.
See full target information MICB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncoimmunology 5:e1048061 PubMed26942056

2016

Failure recovery of circulating NKG2DCD56NK cells in HBV-associated hepatocellular carcinoma after hepatectomy predicts early recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Gao,Zhaojun Duan,Ling Zhang,Xiangbo Huang,Lu Long,Jing Tu,Hua Liang,Yu Zhang,Tao Shen,Fengmin Lu

BMC infectious diseases 15:25 PubMed25626490

2015

Soluble MICB protein levels and platelet counts during hepatitis B virus infection and response to hepatocellular carcinoma treatment.

Applications

sELISA

Species

Human

Hoang Van Tong,Le Huu Song,Nghiem Xuan Hoan,Bui Khac Cuong,Bui Tien Sy,Ho Anh Son,Do Quyet,Vu Quoc Binh,Peter G Kremsner,Claus Thomas Bock,Thirumalaisamy P Velavan,Nguyen Linh Toan

Oncoimmunology 4:e991228 PubMed25949910

2015

Metabolic perturbation sensitizes human breast cancer to NK cell-mediated cytotoxicity by increasing the expression of MHC class I chain-related A/B.

Applications

Unspecified application

Species

Unspecified reactive species

Dexue Fu,Jean-Francois Geschwind,Swathi Karthikeyan,Eliyahu Miller,Rani Kunjithapatham,Zhijun Wang,Shanmugasundaram Ganapathy-Kanniappan
View all publications
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