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AB100594

Human MIF ELISA Kit

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(3 Publications)

Human MIF ELISA Kit is a sandwich ELISA designed to quantify Human MIF with a sensitivity of 6 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 8.23 - 6000 pg/mL

View Alternative Names

GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF

6 Images
ELISA - Human MIF ELISA Kit (AB100594)
  • ELISA

Lab

ELISA - Human MIF ELISA Kit (AB100594)

MIF in THP1 supernatants from control cells or cells stimulated (P/L) for 24 hours with 50 ng x mL-1 PMA (ab120297) and 1 ug x mL-1 LPS for the last 6 hours. Samples tested in the range of 1/3-1/30 (duplicates, +/- SD).

ELISA - Human MIF ELISA Kit (AB100594)
  • ELISA

Lab

ELISA - Human MIF ELISA Kit (AB100594)

MIF measured in cell culture supernatants from control or treated (PHA : 2 days in 2% PHA-M; LifeTechnologies) PBMCs. Samples tested in the range of 1/3-1/30 (duplicates, +/- SD).

ELISA - Human MIF ELISA Kit (AB100594)
  • ELISA

Lab

ELISA - Human MIF ELISA Kit (AB100594)

MIF in human biological fluids (duplicates +/- SD). Data shown from undiluted serum, plasma and urine, and milk diuted 1/100-1/500. Mouse serum gave 0.12 ng/mL (duplicates) and no signal was detected in mouse plasma.

ELISA - Human MIF ELISA Kit (AB100594)
  • ELISA

Lab

ELISA - Human MIF ELISA Kit (AB100594)

Standard curve in assay buffer B with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Human MIF ELISA Kit (AB100594)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIF ELISA Kit (AB100594)

Representative Standard Curve using ab100594.

Sandwich ELISA - Human MIF ELISA Kit (AB100594)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIF ELISA Kit (AB100594)

Representative Standard Curve using ab100594.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MIF ELISA Kit ab100594 is a sandwich ELISA to measure Human MIF in serum, plasma, cell culture supernatant with a sensitivity of 6 pg/ml.

How the assay works

This assay employs an antibody specific for Human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human MIF ELISA Kit ab100594 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 103 %", "average": "= 94.68" }, { "sample": "Serum", "range": "82 - 102 %", "average": "= 93.48" }, { "sample": "Plasma", "range": "84 - 103 %", "average": "= 95.41" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100594-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100594-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100594-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100594-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100594-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100594-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human MIF", "number":"AB100594-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human MIF Standard (lyophilized)", "number":"AB100594-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MIF Microplate (12 x 8 wells)", "number":"AB100594-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100594-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.
Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.
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Product protocols

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Target data

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 11439086, PubMed : 17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 11439086, PubMed : 17526494).
See full target information MIF
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3620 PubMed37365178

2023

Single-cell transcriptomics and epigenomics unravel the role of monocytes in neuroblastoma bone marrow metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Irfete S Fetahu,Wolfgang Esser-Skala,Rohit Dnyansagar,Samuel Sindelar,Fikret Rifatbegovic,Andrea Bileck,Lukas Skos,Eva Bozsaky,Daria Lazic,Lisa Shaw,Marcus Tötzl,Dora Tarlungeanu,Marie Bernkopf,Magdalena Rados,Wolfgang Weninger,Eleni M Tomazou,Christoph Bock,Christopher Gerner,Ruth Ladenstein,Matthias Farlik,Nikolaus Fortelny,Sabine Taschner-Mandl

Scientific reports 11:8632 PubMed33883602

2021

Prediction of sarcopenia using a battery of circulating biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Asima Karim,Tahir Muhammad,Islam Shah,Javaidullah Khan

Nature cell biology 17:816-26 PubMed25985394

2015

Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno Costa-Silva,Nicole M Aiello,Allyson J Ocean,Swarnima Singh,Haiying Zhang,Basant Kumar Thakur,Annette Becker,Ayuko Hoshino,Milica Tešić Mark,Henrik Molina,Jenny Xiang,Tuo Zhang,Till-Martin Theilen,Guillermo García-Santos,Caitlin Williams,Yonathan Ararso,Yujie Huang,Gonçalo Rodrigues,Tang-Long Shen,Knut Jørgen Labori,Inger Marie Bowitz Lothe,Elin H Kure,Jonathan Hernandez,Alexandre Doussot,Saya H Ebbesen,Paul M Grandgenett,Michael A Hollingsworth,Maneesh Jain,Kavita Mallya,Surinder K Batra,William R Jarnagin,Robert E Schwartz,Irina Matei,Héctor Peinado,Ben Z Stanger,Jacqueline Bromberg,David Lyden
View all publications
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websiteProtocolBooklet
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