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AB214569

Human MIP-1 alpha/CCL3 ELISA Kit

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(1 Publication)

Human MIP-1 alpha/CCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MIP-1 alpha/CCL3 with a sensitivity of 0.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha

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Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1a concentration was determined to be 5.5 pg/mL with a range of 1.3 – 12.1 pg/mL.

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)

Interpolated concentrations of native MIP1a in human peripheral blood monocyte (PBMC) cell culture supernatant samples.

The concentrations of MIP1a were measured in duplicates, interpolated from the MIP1a standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated 6.35% and stimulated 0.2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1a concentration was determined to be 0.241 ng/mL in unstimulated PBMC cell culture supernatant and 40.8 ng/mL in stimulated.

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)
  • sELISA

Unknown

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)

Human MIP1a standard curve comparison data.

Standard curve comparison between human MIP1a SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 24-fold increase in sensitivity.

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)

Interpolated concentrations of spiked MIP1a in human serum and plasma samples.

The concentrations of MIP1a were measured in duplicates, interpolated from the MIP1a standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%) and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit (AB214569)

Example of MIP1a standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.4 pg/mL

Range

1.56 - 100 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MIP-1 alpha/CCL3 ELISA Kit (ab214569) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP-1 alpha/CCL3 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MIP-1 alpha/CCL3 with 0.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Macrophage Inflammatory Protein 1-alpha (MIP1a, also known as CCL3) is a monokine with both inflammatory and chemokine properties. MIP-1-alpha can bind to CCR1, CCR4 and CCR5. In addition, it is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 102 %", "average": "= 101" }, { "sample": "Serum", "range": "96 - 101 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "92 - 105 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "94 - 100 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "91 - 101 %", "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.
Biological function summary

Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.

Pathways

MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.

MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.
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Product protocols

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Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
See full target information CCL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 26:97 PubMed33121429

2020

IP-10 and MCP-1 as biomarkers associated with disease severity of COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Jinglan Wang,Chenxi Liu,Longxiang Su,Dong Zhang,Junping Fan,Yanli Yang,Meng Xiao,Jing Xie,Yingchun Xu,Yongzhe Li,Shuyang Zhang
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