AB269562

Human MIP-3 alpha ELISA Kit (CCL20)

Recombinant
SimpleStep
What is this?

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(4 Publications)

Human MIP-3 alpha ELISA Kit (CCL20) is a single-wash 90-min Simplestep used to quantify Human MIP-3 alpha (CCL20) with a sensitivity of 5.11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

LARC, MIP3A, SCYA20, CCL20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha

4 Images

ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

ELISA

Supplier Data

ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

sELISA

Supplier Data

Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

Interpolated concentrations of native MIP-3 alpha in human cell culture supernatant samples.

The concentrations of MIP-3 alpha were measured in duplicates, interpolated from the MIP-3 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated PBMC 100%, 1.5% PHA stimulated PBMC cell culture supernatant 50%, unstimulated THP-1 100%, and 1ug/mL IFN-gamma and 1ug/mL LPS stimulated THP-1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP-3 alpha concentration was determined to be undetectable in unstimulated PBMC supernatant, 1614.14 pg/mL in PHA stimulated PBMC, undetectable in unstimulated THP-1 supernatant, and 1209.37 pg/mL in IFN-gamma and LPS stimulated THP-1 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

Human MIP-3 standard curve comparison.

Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab178015) and current human MMP3 SimpleStep ELISA kit (ab269562).

sELISA

Supplier Data

Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

Example of human MIP-3 alpha standard curve in Sample Diluent NS.

The MIP-3 alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.11 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MIP-3 alpha ELISA Kit (CCL20) ab269562 is a rapid single-wash 90-min Sandwich ELISA to measure Human MIP-3 alpha (CCL20)in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 5.11 pg/mL.

How the assay works

Human MIP-3 alpha (CCL20)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MIP-3 alpha ELISA Kit (CCL20) ab269562 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

MIP-3 alpha (MIP3a, CCL20) is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells. Its main function is to inhibit proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3 alpha is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. There are also low levels in thymus, prostate, testis, small intestine and colon. MIP-3 alpha is induced by bacterial lipopolysaccharides, TNF and IFNG/IFN-gamma. Stimulation with phorbol myristate acetate (PMA) in U-937 cell line and Bowes melanoma will induce expression. MIP-3 alpha shares 64.2%, 60.8%, 69.8%, 86.5% sequence homology with mouse, rat, cow, and monkey, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 92" }, { "sample": "Serum", "range": "83 - 90 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "83 - 90 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "88 - 106 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "84 - 93 %", "average": "= 96" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269562-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269562-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269562-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269562-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269562-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269562-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MIP-3 alpha (CCL20) Lyophilized Recombinant Protein", "number":"AB269562-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MIP-3 alpha (CCL20) Capture Antibody", "number":"AB269562-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB269562-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269562-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MIP-3 alpha (CCL20) Detector Antibody", "number":"AB269562-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha) also known as CCL20 is a chemokine with a molecular mass of approximately 9 kDa. It plays a role in immune response by mediating chemotaxis of lymphocytes and dendritic cells. MIP-3 alpha is mainly expressed in liver lung and lymphoid organs including the lymph nodes and appendix. Its expression can be induced during inflammation in response to signaling molecules like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1?).

Biological function summary

MIP-3 alpha contributes to the immune system by recruiting immune cells to sites of inflammation or injury. It is not part of a larger protein complex. Its main function is binding to the CCR6 receptor on target cells. This interaction mediates the migration and activation of the immune cells critical for initiating the immune response by increasing cell surface adhesion and motility. By drawing immune cells MIP-3 alpha significantly influences the body's capacity to fight infections and regulate inflammatory processes.

Pathways

MIP-3 alpha is integral to the inflammatory response and chemokine signaling pathways. It is involved in the immune system's adaptive response through its interaction with CCR6. This pathway overlaps with other chemokines such as MIP-1 and MIP-2 which also aid in immune cell recruitment. Furthermore MIP-3 alpha’s engagement in these pathways facilitates intercellular communication during immune responses pivotal for maintaining homeostasis and immune surveillance.

MIP-3 alpha has associations with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. During these conditions elevated levels of MIP-3 alpha contribute to the recruitment of inflammatory cells which exacerbate symptoms. MIP-3 alpha also connects with other proteins such as TNF-alpha in these disease contexts enhancing inflammation and progression of these disorders. Targeting MIP-3 alpha and its associated pathways may provide therapeutic opportunities for alleviating these chronic inflammatory conditions.

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Product protocols

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Target data

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 11035086, PubMed : 11352563, PubMed : 20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases (PubMed : 21376174). CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (PubMed : 11352563, PubMed : 9038201). Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (By similarity). C-terminal processed forms have been shown to be equally chemotactically active for leukocytes (PubMed : 11035086). Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed : 23765988, PubMed : 25122636). Inhibits proliferation of myeloid progenitors in colony formation assays (PubMed : 9129037). May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (By similarity). Possesses antibacterial activity towards E.coli ATCC 25922 and S.aureus ATCC 29213 (PubMed : 12149255).

See full target information CCL20

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1216376 PubMed37398643

2023

Proteasome-dependent senescent tumor cells mediate immunosuppression through CCL20 secretion and M2 polarization in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengwei Wu,Jiashu Han,Hao Wu,Ziwen Liu

EMBO reports 23:e55871 PubMed36200589

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Walter Wahli,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Journal of translational medicine 20:248 PubMed35642002

2022

Role of chemokine-mediated angiogenesis in resistance towards crizotinib and its reversal by anlotinib in EML4-ALK positive NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Wang,Ning Lou,Rongrong Luo,Xuezhi Hao,Yutao Liu,Lin Wang,Yuankai Shi,Xiaohong Han

EMBO reports 23:e54271 PubMed35403791

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MIP-3 alpha ELISA Kit (CCL20)

ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (AB269562)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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