Human MIP-3 alpha ELISA Kit (CCL20) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MIP-3 alpha (CCL20) in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Frontiers in immunology 14:12163762023Proteasome-dependent senescent tumor cells mediate immunosuppression through CCL20 secretion and M2 polarization in pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengwei Wu,Jiashu Han,Hao Wu,Ziwen LiuPubMed 37398643
- EMBO reports 23:e558712022Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Walter Wahli,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine CheungPubMed 36200589
- Journal of translational medicine 20:2482022Role of chemokine-mediated angiogenesis in resistance towards crizotinib and its reversal by anlotinib in EML4-ALK positive NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShasha Wang,Ning Lou,Rongrong Luo,Xuezhi Hao,Yutao Liu,Lin Wang,Yuankai Shi,Xiaohong HanPubMed 35642002
- EMBO reports 23:e542712022Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine CheungPubMed 35403791
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.11 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:11035086, PubMed:11352563, PubMed:20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases (PubMed:21376174). CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (PubMed:11352563, PubMed:9038201). Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (By similarity). C-terminal processed forms have been shown to be equally chemotactically active for leukocytes (PubMed:11035086). Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:23765988, PubMed:25122636). Inhibits proliferation of myeloid progenitors in colony formation assays (PubMed:9129037). May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (By similarity). Possesses antibacterial activity towards E.coli ATCC 25922 and S.aureus ATCC 29213 (PubMed:12149255).
Alternative names
LARC, MIP3A, SCYA20, CCL20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha
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Human MIP-3 alpha ELISA Kit (CCL20) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MIP-3 alpha (CCL20) in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 18.75 - 1200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5.11 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 3.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 92 | Range 84 - 104 % |
Sample type Serum | Average % = 86 | Range 83 - 90 % |
Sample type EDTA Plasma | Average % = 88 | Range 83 - 90 % |
Sample type Heparin Plasma | Average % = 98 | Range 88 - 106 % |
Sample type Citrate plasma | Average % = 96 | Range 84 - 93 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human MIP-3 alpha ELISA Kit (CCL20) (ab269562) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP-3 alpha (CCL20) protein in cell culture supernatant, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MIP-3 alpha (CCL20) with 5.11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MIP-3 alpha (MIP3a, CCL20) is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells. Its main function is to inhibit proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3 alpha is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. There are also low levels in thymus, prostate, testis, small intestine and colon. MIP-3 alpha is induced by bacterial lipopolysaccharides, TNF and IFNG/IFN-gamma. Stimulation with phorbol myristate acetate (PMA) in U-937 cell line and Bowes melanoma will induce expression. MIP-3 alpha shares 64.2%, 60.8%, 69.8%, 86.5% sequence homology with mouse, rat, cow, and monkey, respectively.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha) also known as CCL20 is a chemokine with a molecular mass of approximately 9 kDa. It plays a role in immune response by mediating chemotaxis of lymphocytes and dendritic cells. MIP-3 alpha is mainly expressed in liver lung and lymphoid organs including the lymph nodes and appendix. Its expression can be induced during inflammation in response to signaling molecules like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1β).
- Biological function summary
MIP-3 alpha contributes to the immune system by recruiting immune cells to sites of inflammation or injury. It is not part of a larger protein complex. Its main function is binding to the CCR6 receptor on target cells. This interaction mediates the migration and activation of the immune cells critical for initiating the immune response by increasing cell surface adhesion and motility. By drawing immune cells MIP-3 alpha significantly influences the body's capacity to fight infections and regulate inflammatory processes.
- Pathways
MIP-3 alpha is integral to the inflammatory response and chemokine signaling pathways. It is involved in the immune system's adaptive response through its interaction with CCR6. This pathway overlaps with other chemokines such as MIP-1 and MIP-2 which also aid in immune cell recruitment. Furthermore MIP-3 alpha’s engagement in these pathways facilitates intercellular communication during immune responses pivotal for maintaining homeostasis and immune surveillance.
- Associated diseases and disorders
MIP-3 alpha has associations with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. During these conditions elevated levels of MIP-3 alpha contribute to the recruitment of inflammatory cells which exacerbate symptoms. MIP-3 alpha also connects with other proteins such as TNF-alpha in these disease contexts enhancing inflammation and progression of these disorders. Targeting MIP-3 alpha and its associated pathways may provide therapeutic opportunities for alleviating these chronic inflammatory conditions.
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3 product images
Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (ab269562)
Human MIP-3 standard curve comparison.
Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab178015) and current human MMP3 SimpleStep ELISA kit (ab269562).
Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (ab269562)
Example of human MIP-3 alpha standard curve in Sample Diluent NS.
The MIP-3 alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MIP-3 alpha ELISA Kit (CCL20) (ab269562)
Interpolated concentrations of native MIP-3 alpha in human cell culture supernatant samples.
The concentrations of MIP-3 alpha were measured in duplicates, interpolated from the MIP-3 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC 100%, 1.5% PHA stimulated PBMC cell culture supernatant 50%, unstimulated THP-1 100%, and 1ug/mL IFN-gamma and 1ug/mL LPS stimulated THP-1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP-3 alpha concentration was determined to be undetectable in unstimulated PBMC supernatant, 1614.14 pg/mL in PHA stimulated PBMC, undetectable in unstimulated THP-1 supernatant, and 1209.37 pg/mL in IFN-gamma and LPS stimulated THP-1 supernatant.
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