Human MIP1 beta ELISA Kit (CCL4) is a Sandwich (quantitative) ELISA kit for the measurement of Human MIP1 beta (CCL4) in Human in Plasma, Cell culture supernatant, Serum samples.
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- Journal of extracellular vesicles 9:18011532020(Sub)populations of extracellular vesicles released by TNF-α -triggered human endothelial cells promote vascular inflammation and monocyte migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaharak Hosseinkhani et. al.PubMed 32944190
- Frontiers in immunology 9:17892018Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaharak Hosseinkhani et. al.PubMed 30131806
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
4.1 - 1000 pg/mL
- Sensitivity
- < 2.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Alternative names
LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2
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Human MIP1 beta ELISA Kit (CCL4) is a Sandwich (quantitative) ELISA kit for the measurement of Human MIP1 beta (CCL4) in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
4.1 - 1000 pg/mL
- Assay Platform
Microplate
- Sensitivity
- < 2.5 pg/mL
- General recovery
95%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 97.42 | Range 85 - 104 % |
Sample type Serum | Average % = 95.64 | Range 82 - 102 % |
Sample type Plasma | Average % = 94.32 | Range 83 - 103 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's MIP1 beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP1 beta in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MIP1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MIP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
This product may also recognise CC4L [C-C motif chemokine 4-like], a family member which shares high homology with CCL4.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.
- Biological function summary
CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.
- Pathways
CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.
- Associated diseases and disorders
CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.
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2 product images
Sandwich ELISA - Human MIP1 beta ELISA Kit (CCL4) (ab100597)
Representative Standard Curve using ab100597.
Sandwich ELISA - Human MIP1 beta ELISA Kit (CCL4) (ab100597)
Representative Standard Curve using ab100597.
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