Human MIP2 ELISA Kit (CXCL2) is a single-wash 90-min Simplestep used to quantify Human MIP2 (CXCL2) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
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- Oncology letters 25:2252023Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Li,Junmei Li,Feng Yu,Bo Wang,Bing LiuPubMed 37153045
- Cellular and molecular gastroenterology and hepatology 15:463-4852022DCLK1 Suppresses Tumor-Specific Cytotoxic T Lymphocyte Function Through Recruitment of MDSCs via the CXCL1-CXCR2 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Yan,Jianjian Li,Zeru Xiao,Xiaona Fan,Heshu Liu,Ying Xu,Ruya Sun,Jian Liu,Jiannan Yao,Guangyu An,Yan Shi,Yang GePubMed 36309200
- Science advances 8:eabo31922022Distinctive exercise-induced inflammatory response and exerkine induction in skeletal muscle of people with type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicolas J Pillon,Jonathon A B Smith,Petter S Alm,Alexander V Chibalin,Julia Alhusen,Erik Arner,Piero Carninci,Tomas Fritz,Julia Otten,Tommy Olsson,Sophie van Doorslaer de Ten Ryen,Louise Deldicque,Kenneth Caidahl,Harriet Wallberg-Henriksson,Anna Krook,Juleen R ZierathPubMed 36070371
- Journal of nanobiotechnology 20:2182022Targeted neutrophil-mimetic liposomes promote cardiac repair by adsorbing proinflammatory cytokines and regulating the immune microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Chen,Yanan Song,Qiaozi Wang,Qiyu Li,Haipeng Tan,Jinfeng Gao,Ning Zhang,Xueyi Weng,Dili Sun,Wusiman Yakufu,Zhengmin Wang,Juying Qian,Zhiqing Pang,Zheyong Huang,Junbo GePubMed 35525963
- Molecular therapy : the journal of the American Society of Gene Therapy 30:672-6872021A chemokine regulatory loop induces cholesterol synthesis in lung-colonizing triple-negative breast cancer cells to fuel metastatic growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingchen Han,Felix Alonso-Valenteen,Zhe Wang,Nan Deng,Tian-Yu Lee,Bowen Gao,Ying Zhang,Yali Xu,Xinfeng Zhang,Sandrine Billet,Xuemo Fan,Stephen Shiao,Neil Bhowmick,Lali Medina-Kauwe,Armando Giuliano,Xiaojiang CuiPubMed 34274535
- Cellular signalling 77:1098252020The SGLT2 inhibitor Empagliflozin attenuates interleukin-17A-induced human aortic smooth muscle cell proliferation and migration by targeting TRAF3IP2/ROS/NLRP3/Caspase-1-dependent IL-1β and IL-18 secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergiy Sukhanov,Yusuke Higashi,Tadashi Yoshida,Srinivas Mummidi,Annayya R Aroor,Jacob Jeffrey Russell,Shawn B Bender,Vincent G DeMarco,Bysani ChandrasekarPubMed 33160017
- British journal of cancer 123:459-4702020Omental adipocytes promote peritoneal metastasis of gastric cancer through the CXCL2-VEGFA axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Natsume et. al.PubMed 32439934
- Toxicological sciences : an official journal of the Society of Toxicology 175:210-2192020Per- and Polyfluoroalkyl Substances Differentially Inhibit Placental Trophoblast Migration and Invasion In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn T Szilagyi,Anastasia N Freedman,Stewart L Kepper,Arjun M Keshava,Jackie T Bangma,Rebecca C FryPubMed 32219433
- The Journal of international medical research 48:3000605198961502020Characteristics of expression in coronary atherosclerosis and negative regulation by microRNA-421.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Yang,Hu Liu,Qian Cao,Wei ZhongPubMed 32212883
- Experimental and therapeutic medicine 19:1297-13032020Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wang,Yupei Ke,Tao ShuPubMed 32010302
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.3 - 150 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- < 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
Alternative names
GRO2, GROB, MIP2A, SCYB2, CXCL2, C-X-C motif chemokine 2, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, Gro-beta, MIP2-alpha
What's included?
Recommended products
Human MIP2 ELISA Kit (CXCL2) is a single-wash 90-min Simplestep used to quantify Human MIP2 (CXCL2) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.3 - 150 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- < 1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 24 | mean - | SD - | C.V. 2.81 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 24 | mean - | SD - | C.V. 3.46 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101.3 | Range 89.9 - 110.09 % |
Sample type Cell culture media | Average % = 106.57 | Range 97.09 - 116.04 % |
Sample type EDTA Plasma | Average % = 96.92 | Range 94.06 - 100.01 % |
Sample type Heparin Plasma | Average % = 100.07 | Range 87.61 - 116.98 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human MIP2 (CXCL2) ELISA kit (ab184862) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP2 protein in in human serum, plasma, and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human MIP2 with 1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Macrophage inflammatory protein 2 (MIP2) otherwise known as CXCL2 GRO-beta| or Hematopoietic synergistic factor is a 7.9 kDa heparin-binding chemokine that has potent effects in the response to inflammation and induction of peripheral tolerance. MIP2 shares 70% sequence similarity with mouse and rat MIP2. It is secreted by activated monocytes neutrophils and inflamed mucosal epithelial cells in response to inflammatory stimuli such as IL-1β. MIP2 recruits granulocytic neutrophils and macrophages at sights of inflammation| and causes degranulation of these effector cells at the inflammatory site. It has also been hypothesized that MIP2 acts to synergize the effects of Granulocyte macrophage colony-stimulating factor (GM-CSF) and Macrophage colony-stimulating factor (M-CSF) leading to a larger recruitment of neutrophils and macrophages at the site of inflammation. Research has also suggested that MIP2 is involved in tolerogenic pathways as a lack of the MIP2 receptors CXCR1 and CXCR2 are associated with peripheral tolerance. Clinically. MIP2 is being used as a bone marrow transplant regimen to mobilize peripheral blood hematopoietic stem cells (PBSCs). Mobilization by MIP2 therapy in combination with G-CSF has been associated with faster immune cell reconstitution after transplantation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
- Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
- Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
- Associated diseases and disorders
CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.
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5 product images
Sandwich ELISA - Human MIP2 ELISA Kit (CXCL2) (ab184862)
Linearity of dilution for biologicals.
Interpolated values corrected by dilution factor (mean +/- SD) are graphed.
Sandwich ELISA - Human MIP2 ELISA Kit (CXCL2) (ab184862)
Example of MIP2 standard curve in Sample Diluent NS using ab184862
Sandwich ELISA - Human MIP2 ELISA Kit (CXCL2) (ab184862)
Example of MIP2 standard curve in Sample Diluent 25BP using ab184862
Sandwich ELISA - Human MIP2 ELISA Kit (CXCL2) (ab184862)
Specificity of MIP2 on stimulated and non stimulated media supernatants.
Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin. Cells were cultured for 2 days at 37˚C in the presence or absence of PHA. The concentrations of MIP2 were interpolated from the calibration curve and corrected for sample dilution. The mean MIP2 concentration was 67 pg/mL on unstimulated PBMC supernatants and 773 pg/mL on stimulated PBMCs supernatants.
Sandwich ELISA - Human MIP2 ELISA Kit (CXCL2) (ab184862)
Levels of MIP2 in human serum samples.
Human MIP2 levels in individual healthy donors. Ten individual healthy donors were evaluated for the presence of MIP2 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent 25BP and corrected for sample dilution (1:4). The mean level of Human MIP2 was found at 84.757 pg/mL with a range of 26.767 – 266.256 pg/mL.
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