Human MIP5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MIP5 with a sensitivity of 27.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Other biological fluids, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 89.4 - 1500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 27.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the CCL15.
Alternative names
MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5
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Human MIP5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MIP5 with a sensitivity of 27.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Other biological fluids, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 89.4 - 1500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 27.1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 3 | mean - | SD - | C.V. 3.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 5 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 104 | Range 97 - 110 % |
Sample type Serum | Average % = 92 | Range 90 - 95 % |
Sample type Other biological fluids | Average % = 117 | Range 116 - 117 % |
Sample type EDTA Plasma | Average % = 107 | Range 102 - 115 % |
Sample type Cell culture extracts | Average % = 106 | Range 100 - 115 % |
Sample type Tissue Extracts | Average % = 108 | Range 91 - 121 % |
Sample type Heparin Plasma | Average % = 108 | Range 102 - 112 % |
Sample type Citrate plasma | Average % = 103 | Range 91 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human MIP5 ELISA Kit (ab217774) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP5 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, other biological fluids, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MIP5 with 27.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MIP5 is a secreted monomeric chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. MIP5 acts mainly via CC chemokine receptor CCR1. It also binds to CCR3. MIP5 can be cleaved into 3 chains, MIP5(22-92), MIP5(25-92) and MIP5(29-92). These cleavage products are more potent chemo-attractants than MIP5. They are also found in high levels in synovial fluids from rheumatoid patients.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Macrophage inflammatory protein 5 (MIP-5) also known as CCL15 is a chemokine involved in immune system function. It plays an important role in recruiting and activating certain immune cells such as monocytes and lymphocytes. Its molecular mass is approximately 13.5 kDa. MIP-5 is produced mainly by monocytes and macrophages and can be found in the liver and several other tissues. This protein mediates inflammation by guiding immune cells to sites of infection or injury where they can perform immune defense activities.
- Biological function summary
MIP-5 is involved in the chemotactic movement of immune cells a process where cells move in response to chemical stimuli. It is not part of a protein complex but interacts dynamically with cell surface receptors to induce cellular responses. MIP-5 triggers cells to release further signaling molecules increasing the inflammatory response's effectiveness. This chemokine creates a highly coordinated immune response by initiating a signaling cascade that helps maintain immune surveillance and response to pathogens.
- Pathways
MIP-5 plays a role in the chemokine signaling pathway where it interacts with specific receptors that lead to cellular migration and activation. It also fits into the cytokine-cytokine receptor interaction pathway influencing a range of cellular responses tied to immune function. MIP-5 shares functional properties with other chemokines like MCP-1 (monocyte chemoattractant protein-1) which similarly attract immune cells and mediate immune responses.
- Associated diseases and disorders
MIP-5 is linked to inflammatory diseases and certain types of cancer. Elevated levels of MIP-5 have been observed in rheumatoid arthritis where it contributes to joint inflammation. It is also associated with hepatocellular carcinoma where it may influence tumor microenvironment and metastasis. In these conditions MIP-5 might interact with other inflammatory molecules such as IL-6 and TNF-alpha suggesting its role as a mediator of chronic inflammation and malignancy processes.
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Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)
Example of human MIP5 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)
Example of human MIP5 standard curve in 1X Cell Extraction Buffer PTR.
The MIP5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)
Interpolated concentrations of native MIP5 in human serum and plasma samples.
The concentrations of MIP5 were measured in duplicates, interpolated from the MIP5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 20%, plasma (heparin) 20% and plasma (EDTA) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 8705 pg/mL in neat serum, 7196 pg/mL in neat plasma (citrate), 6848 pg/mL in neat plasma (heparin), and 7231 pg/mL in neat plasma (EDTA).
Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)
Interpolated concentrations of native MIP5 in HepG2 (4 days) cell culture supernatant and human synovial fluid samples.
The concentrations of MIP5 were measured in duplicates, interpolated from the MIP5 standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 100% (neat), and synovial fluid 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 706.2 pg/mL in neat HepG2 cell culture supernatant and 5,385 pg/mL in neat synovial fluid.
Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)
Interpolated concentrations of native MIP5 in HT-29 cell extract based on a 300 μg/mL extract load and in human colon tissue extract based on a 500 μg/mL extract load.
The concentrations of MIP5 were measured in duplicate and interpolated from the MIP5 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 1293 pg/mL in HT-29 cell extract and 685.5 pg/mL in colon tissue extract.
Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)
Serum from nine individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP5 concentration was determined to be 7978 pg/mL with a range of 3361 – 13894 pg/mL.
Sandwich ELISA - Human MIP5 ELISA Kit (ab217774)
MIP5 is increased in serum of patients with rheumatoid arthritis.
Serum was pooled from fifty apparently healthy donors and compared to serum from a patient who tested positive for rheumatoid factor (RF value 406.2 IU/mL). The concentrations of MIP5 were measured from serum samples in 3 different dilutions in duplicates and interpolated from the MIP5 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean MIP5 concentration was determined to be 8753 pg/mL in healthy human serum and 26193 pg/mL in rheumatoid arthritis patient serum.
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