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AB100598

Human MIP5 ELISA Kit (CCL15)

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(1 Publication)

Human MIP5 ELISA Kit (CCL15) is a Sandwich ELISA for the measurement of Human MIP5 (CCL15) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5

2 Images
Sandwich ELISA - Human MIP5 ELISA Kit (CCL15) (AB100598)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIP5 ELISA Kit (CCL15) (AB100598)

Representative Standard Curve using ab100598.

Sandwich ELISA - Human MIP5 ELISA Kit (CCL15) (AB100598)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIP5 ELISA Kit (CCL15) (AB100598)

Representative Standard Curve using ab100598.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MIP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP5 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human MIP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 112 %", "average": "= 101.5" }, { "sample": "Serum", "range": "92 - 115 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "90 - 114 %", "average": "= 98.23" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100598-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100598-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100598-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100598-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100598-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MIP5 Microplate (12 strips x 8 wells)", "number":"AB100598-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100598-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human MIP5", "number":"AB100598-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100598-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human MIP5 Standard (lyophilized)", "number":"AB100598-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage inflammatory protein 5 (MIP-5) also known as CCL15 is a chemokine involved in immune system function. It plays an important role in recruiting and activating certain immune cells such as monocytes and lymphocytes. Its molecular mass is approximately 13.5 kDa. MIP-5 is produced mainly by monocytes and macrophages and can be found in the liver and several other tissues. This protein mediates inflammation by guiding immune cells to sites of infection or injury where they can perform immune defense activities.
Biological function summary

MIP-5 is involved in the chemotactic movement of immune cells a process where cells move in response to chemical stimuli. It is not part of a protein complex but interacts dynamically with cell surface receptors to induce cellular responses. MIP-5 triggers cells to release further signaling molecules increasing the inflammatory response's effectiveness. This chemokine creates a highly coordinated immune response by initiating a signaling cascade that helps maintain immune surveillance and response to pathogens.

Pathways

MIP-5 plays a role in the chemokine signaling pathway where it interacts with specific receptors that lead to cellular migration and activation. It also fits into the cytokine-cytokine receptor interaction pathway influencing a range of cellular responses tied to immune function. MIP-5 shares functional properties with other chemokines like MCP-1 (monocyte chemoattractant protein-1) which similarly attract immune cells and mediate immune responses.

MIP-5 is linked to inflammatory diseases and certain types of cancer. Elevated levels of MIP-5 have been observed in rheumatoid arthritis where it contributes to joint inflammation. It is also associated with hepatocellular carcinoma where it may influence tumor microenvironment and metastasis. In these conditions MIP-5 might interact with other inflammatory molecules such as IL-6 and TNF-alpha suggesting its role as a mediator of chronic inflammation and malignancy processes.
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Product protocols

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Target data

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the CCL15.
See full target information CCL15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 22:5339-5347 PubMed33174007

2020

Endothelial cell‑derived CCL15 mediates the transmigration of fibrocytes through the CCL15‑CCR1 axis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Pang,Zhixiao Lin,Xiaolin Wang,Lirong Xu,Xiaoli Xu,Rong Huang,Xingxing Li,Xueyong Li,Jinqing Li
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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