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AB267622

Human MIS RII ELISA Kit (AMHR2)

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Human MIS RII ELISA Kit (AMHR2) is a Sandwich (quantitative) ELISA for the measurement of Human MIS RII (AMHR2) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

AMHR, MISR2, AMHR2, Anti-Muellerian hormone type-2 receptor, Anti-Muellerian hormone type II receptor, MIS type II receptor, AMH type II receptor, MISRII, MRII

1 Images
Sandwich ELISA - Human MIS RII ELISA Kit (AMHR2) (AB267622)
  • sELISA

Supplier Data

Sandwich ELISA - Human MIS RII ELISA Kit (AMHR2) (AB267622)

These standard curves are for demonstration only. A standard curve must be run with each assay

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.05 ng/mL

Range

0.041 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human MIS RII ELISA Kit is designed for the quantitative determination of MIS RII in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human MIS RII coated on a 96-well plate. Standards and samples are pipetted into the wells and MIS RII present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIS RII antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIS RII bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand (TNFSF9), Activin RIIB, Aminopeptidase P2 (XPNPEP2), BAMBI (NMA), BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP1, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1(FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, Neurexin 3.

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

Recovery

[ { "sample": "Serum", "range": "86 - 102 %", "average": "= 93.49" }, { "sample": "Plasma", "range": "70 - 82 %", "average": "= 75.52" }, { "sample": "Cell culture media", "range": "85 - 106 %", "average": "= 95.52" } ]

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.
See full target information AMHR2
websiteProtocolBooklet
en

Product promise

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