Human MMP1 ELISA Kit, colorimetric, 90-min ELISA (ab215083)

Human MMP1 ELISA Kit ab215083 is a single-wash 90-min SimpleStep ELISA used to quantify Human MMP1 with a sensitivity of 19.64 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Human MMP1 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation

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Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 93.75 - 6000 pg/mL
Assay time: 1h 30m
Sensitivity: = 19.64 pg/mL

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Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

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Target data

See full target information MMP1

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:1645757, PubMed:2153297, PubMed:2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).

Alternative names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

What's included?

1 x 384 Tests

Components

10X Human MMP1 Capture Antibody

1 x 600 µL

10X Human MMP1 Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

50X Cell Extraction Enhancer Solution (ab193971)

1 x 5 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent CPI2

1 x 6 mL

Human MMP1 Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent 50BS

1 x 20 mL

Sample Diluent NS (ab193972)

1 x 50 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 93.75 - 6000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 19.64 pg/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Overall	n 8	mean -	SD -	C.V. 4

Inter assay

Sample	n	mean	SD	C.V.
Sample Overall	n 3	mean -	SD -	C.V. 2.8

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 103	Range 91 - 114 %
Sample type Serum	Average % = 106	Range 98 - 117 %
Sample type Cell culture extracts	Average % = 104	Range 96 - 111 %
Sample type Heparin Plasma	Average % = 95	Range 93 - 100 %
Sample type Citrate plasma	Average % = 94	Range 89 - 101 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human MMP1 ELISA Kit (ab215083) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP1 protein in cit plasma, hep plasma, serum, cell culture extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP1 with 19.64 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MMP1 is a member of the matrix metalloproteinase family of proteins. These proteins function in the breakdown of the extracellular matrix during a variety of normal and pathological cellular process. MMP1 is an interstitial and fibroblast collagenase.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.

Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

Associated diseases and disorders

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.

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10 product images

Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in Sample Diluent 50BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Interpolated concentrations of native MMP1 in SW480 cell culture supernatant samples.
The concentrations of MMP1 were measured in duplicates, interpolated from the MMP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: SW480 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 12.32 ng/mL in SW480 supernatant.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 1.65 ng/mL with a range of 0.612 – 3.22 ng/mL.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 1.15 ng/mL with a range of 0.589 – 2.17 ng/mL.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Interpolated concentration of native MMP1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% SW480 supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.