Human MMP1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MMP1 in Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.
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Publications
- International journal of molecular medicine 52:2023Exosomes derived from human dermal fibroblasts protect against UVB‑induced skin photoaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Yeon Park,Jung Ok Lee,Youna Jang,Yu-Jin Kim,Jung Min Lee,Su-Young Kim,Beom Joon Kim,Kwang Ho YooPubMed 37888610
- Science signaling 16:eadf95352023The Ca channel ORAI1 is a regulator of oral cancer growth and nociceptive pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGa-Yeon Son,Nguyen Huu Tu,Maria Daniela Santi,Santiago Loya Lopez,Guilherme H Souza Bomfim,Manikandan Vinu,Fang Zhou,Ariya Chaloemtoem,Rama Alhariri,Youssef Idaghdour,Rajesh Khanna,Yi Ye,Rodrigo S LacruzPubMed 37669398
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
93.75 - 6000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 19.64 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:2557822, PubMed:2153297, PubMed:1645757). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).
Alternative names
Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, CLG, MMP1
What's included?
Recommended products
Human MMP1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MMP1 in Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.
Alternative names
Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, CLG, MMP1
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
93.75 - 6000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 19.64 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 2.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 103 | Range 91 - 114 % |
Sample type Serum | Average % = 106 | Range 98 - 117 % |
Sample type Cell culture extracts | Average % = 104 | Range 96 - 111 % |
Sample type Heparin Plasma | Average % = 95 | Range 93 - 100 % |
Sample type Citrate plasma | Average % = 94 | Range 89 - 101 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human MMP1 ELISA Kit (ab215083) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP1 protein in cit plasma, hep plasma, serum, cell culture extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP1 with 19.64 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MMP1 is a member of the matrix metalloproteinase family of proteins. These proteins function in the breakdown of the extracellular matrix during a variety of normal and pathological cellular process. MMP1 is an interstitial and fibroblast collagenase.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- Activity summary
MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.
- Biological function summary
MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.
- Pathways
Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.
- Associated diseases and disorders
MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.
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6 product images
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in Sample Diluent 50BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Example of human MMP1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Interpolated concentrations of native MMP1 in SW480 cell culture supernatant samples.
The concentrations of MMP1 were measured in duplicates, interpolated from the MMP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: SW480 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 12.32 ng/mL in SW480 supernatant.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 1.65 ng/mL with a range of 0.612 – 3.22 ng/mL.
Sandwich ELISA - Human MMP1 ELISA Kit (ab215083)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 1.15 ng/mL with a range of 0.589 – 2.17 ng/mL.
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