AB255715

Human MMP12 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human MMP12 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MMP12 with a sensitivity of 54.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HME, MMP12, Macrophage metalloelastase, MME, Macrophage elastase, Matrix metalloproteinase-12, ME, hME, MMP-12

3 Images

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

sELISA

Supplier Data

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

Interpolated concentrations of native MMP12 in human spleen extract based on a 3000 μg/mL extract load.

The concentrations of MMP12 were measured in duplicate and interpolated from the MMP12 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP12 concentration was determined to be 7556.82 pg/mL in spleen extract.

sELISA

Supplier Data

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

Representative image of Human MMP12 ELISA Kit (ab255715)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human MMP12 ELISA Kit Capture Antibody

- 10X Human MMP12 Detector Antibody

- 10X Human MMP12 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human MMP12 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

Example of human MMP12 standard curve in 1X Cell Extraction Buffer PTR.

The MMP12 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 54.9 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human MMP12 ELISA Kit (ab255715) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP12 protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP12 with 54.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MMP12 (Matrix metalloproteinase-12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. MMP12 is member of a cluster of MMP genes that localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. MMP12 may play a role in aneurysm formation and in tissue injury and remodeling. Studies in mice suggest a role in the development of emphysema. Human MMP12 shares 62% and 60% sequence homology with mouse and rat respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "81 - 104 %", "average": "= 96" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab255715-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB255715-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB255715-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB255715-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB255715-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB255715-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB255715-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB255715-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB255715-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB255715-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MMP12 Lyophilized Recombinant Protein", "number":"AB255715-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MMP12 Capture Antibody", "number":"AB255715-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MMP12 Detector Antibody", "number":"AB255715-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.

Biological function summary

MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.

Pathways

MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.

Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.

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Product protocols

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Target data

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

See full target information MMP12

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 57:e13351 PubMed38511770

2024

Silencing of matrix metalloprotease-12 delays the progression of castration-resistant prostate cancer by regulating autophagy and lipolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zheng,Xiaoqin Xie,Wei Wang,Liang Wang,Bing Tan

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human MMP12 ELISA Kit

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

Sandwich ELISA - Human MMP12 ELISA Kit (AB255715)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise