Human MMP13 ELISA Kit is a sandwich ELISA designed to quantify Human MMP13 with a sensitivity of 6 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 8.23 - 6000 pg/mL
- Cited in over 20 publications
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Publications
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- Nature 625:557-5652024Na1.7 as a chondrocyte regulator and therapeutic target for osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Fu,Dmytro Vasylyev,Yufei Bi,Mingshuang Zhang,Guodong Sun,Asya Khleborodova,Guiwu Huang,Libo Zhao,Renpeng Zhou,Yonggang Li,Shujun Liu,Xianyi Cai,Wenjun He,Min Cui,Xiangli Zhao,Aubryanna Hettinghouse,Julia Good,Ellen Kim,Eric Strauss,Philipp Leucht,Ran Schwarzkopf,Edward X Guo,Jonathan Samuels,Wenhuo Hu,Mukundan Attur,Stephen G Waxman,Chuan-Ju LiuPubMed 38172636
- Antioxidants (Basel, Switzerland) 12:2023Matrix Metalloproteinase 13 Is Associated with Age-Related Choroidal Neovascularization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJorge González-Zamora,María Hernandez,Sergio Recalde,Jaione Bezunartea,Ana Montoliu,Valentina Bilbao-Malavé,Sara Llorente-González,Alfredo García-Layana,Patricia Fernández-RobredoPubMed 37107259
- Mediators of inflammation 2022:42587422022ST3GAL3 Promotes the Inflammatory Response of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Activating the TLR9/MyD88 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiming Xu,Xuegang Niu,Yifan Liu,Lining LiuPubMed 36405992
- Toxins 13:2021Crotoxin Modulates Events Involved in Epithelial-Mesenchymal Transition in 3D Spheroid Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEllen Emi Kato,Sandra Coccuzzo SampaioPubMed 34822613
- Environmental toxicology 36:2448-24532021Oxymatrine hydrazone (OMTH) synthesis and its protective effect for rheumatoid arthritis through downregulation of MEK/NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangwen Zhang,Baojian Liu,Zhaoyang Zeng,Qinghai Chen,Yiyun Feng,Xueqian NingPubMed 34459104
- ACS omega 6:2149-21552021Roflumilast Reduced the IL-18-Induced Inflammatory Response in Fibroblast-Like Synoviocytes (FLS).Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Zhong et. al.PubMed 33521454
- Medical science monitor : international medical journal of experimental and clinical research 26:e9247872020Retinoic Acid-Platinum (II) Complex [RT-Pt(II)] Protects Against Rheumatoid Arthritis in Mice via MEK/Nuclear Factor kappa B (NF-κB) Pathway Downregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqiang Cui,Yaping Lin,Yuhong Liu,Ling Cao,Li CuiPubMed 32741960
- Nature communications 10:54102019Thyroid hormone induces progression and invasiveness of squamous cell carcinomas by promoting a ZEB-1/E-cadherin switch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaterina Miro et. al.PubMed 31776338
- International immunopharmacology 76:1058392019MicroRNA-27a alleviates IL-1β-induced inflammatory response and articular cartilage degradation via TLR4/NF-κB signaling pathway in articular chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Jun Qiu et. al.PubMed 31520995
- Cancer management and research 11:7439-74532019Down-regulation of CCL17 in cancer-associated fibroblasts inhibits cell migration and invasion of breast cancer through ERK1/2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjie Li et. al.PubMed 31496803
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8.23 - 6000 pg/mL
- Sensitivity
- < 6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Alternative names
Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13
What's included?
Recommended products
Human MMP13 ELISA Kit is a sandwich ELISA designed to quantify Human MMP13 with a sensitivity of 6 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 8.23 - 6000 pg/mL
- Cited in over 20 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 8.23 - 6000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 6 pg/mL
- General recovery
- 93%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 94.72 | Range 84 - 103 % |
Sample type Plasma | Average % = 93.13 | Range 83 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's MMP13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MMP13 in plasma and cell culture supernatants.
This assay employs an antibody specific for Human MMP13 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The kit detects total MMP-13 levels.
We have not been able to detect endogenous Human MMP13 in normal serum with ab100605, only in serum spiked with Human MMP13.
Optimisation may be required with urine samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
- Biological function summary
Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.
- Pathways
Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.
- Associated diseases and disorders
Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.
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2 product images
ELISA - Human MMP13 ELISA Kit (ab100605)
MMP13 measured in cell supernatanses showing quantity (pg) per mL of tested sample
Sandwich ELISA - Human MMP13 ELISA Kit (ab100605)
Representative standard curve using ab100605
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