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AB100605

Human MMP13 ELISA Kit

4

(1 Review)

|

(23 Publications)

Human MMP13 ELISA Kit is a sandwich ELISA designed to quantify Human MMP13 with a sensitivity of 6 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 8.23 - 6000 pg/mL
- Cited in over 20 publications

View Alternative Names

Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13

2 Images
Sandwich ELISA - Human MMP13 ELISA Kit (AB100605)
  • sELISA

Unknown

Sandwich ELISA - Human MMP13 ELISA Kit (AB100605)

Representative standard curve using ab100605

ELISA - Human MMP13 ELISA Kit (AB100605)
  • ELISA

Lab

ELISA - Human MMP13 ELISA Kit (AB100605)

MMP13 measured in cell supernatanses showing quantity (pg) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MMP13 ELISA Kit ab100605 is a sandwich ELISA to measure Human MMP13 in plasma, cell culture supernatant with a sensitivity of 6 pg/ml.

How the assay works

This assay employs an antibody specific for Human MMP13 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human MMP13 ELISA Kit ab100605 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimisation may be required with urine samples.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 94.72" }, { "sample": "Plasma", "range": "83 - 103 %", "average": "= 93.13" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
Biological function summary

Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.

Pathways

Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.

Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.
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Product protocols

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Target data

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
See full target information MMP13
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Nature 625:557-565 PubMed38172636

2024

Na1.7 as a chondrocyte regulator and therapeutic target for osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Fu,Dmytro Vasylyev,Yufei Bi,Mingshuang Zhang,Guodong Sun,Asya Khleborodova,Guiwu Huang,Libo Zhao,Renpeng Zhou,Yonggang Li,Shujun Liu,Xianyi Cai,Wenjun He,Min Cui,Xiangli Zhao,Aubryanna Hettinghouse,Julia Good,Ellen Kim,Eric Strauss,Philipp Leucht,Ran Schwarzkopf,Edward X Guo,Jonathan Samuels,Wenhuo Hu,Mukundan Attur,Stephen G Waxman,Chuan-Ju Liu

Antioxidants (Basel, Switzerland) 12: PubMed37107259

2023

Matrix Metalloproteinase 13 Is Associated with Age-Related Choroidal Neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge González-Zamora,María Hernandez,Sergio Recalde,Jaione Bezunartea,Ana Montoliu,Valentina Bilbao-Malavé,Sara Llorente-González,Alfredo García-Layana,Patricia Fernández-Robredo

Mediators of inflammation 2022:4258742 PubMed36405992

2022

ST3GAL3 Promotes the Inflammatory Response of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Activating the TLR9/MyD88 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Xu,Xuegang Niu,Yifan Liu,Lining Liu

Toxins 13: PubMed34822613

2021

Crotoxin Modulates Events Involved in Epithelial-Mesenchymal Transition in 3D Spheroid Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ellen Emi Kato,Sandra Coccuzzo Sampaio

Environmental toxicology 36:2448-2453 PubMed34459104

2021

Oxymatrine hydrazone (OMTH) synthesis and its protective effect for rheumatoid arthritis through downregulation of MEK/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guangwen Zhang,Baojian Liu,Zhaoyang Zeng,Qinghai Chen,Yiyun Feng,Xueqian Ning

ACS omega 6:2149-2155 PubMed33521454

2021

Roflumilast Reduced the IL-18-Induced Inflammatory Response in Fibroblast-Like Synoviocytes (FLS).

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhong,Shuanghong Guo,Zhongai Yang,Li Han,Junsheng Du,Jin Chen,Xianli Dun,Guangyong Wang

Medical science monitor : international medical journal of experimental and clinical research 26:e924787 PubMed32741960

2020

Retinoic Acid-Platinum (II) Complex [RT-Pt(II)] Protects Against Rheumatoid Arthritis in Mice via MEK/Nuclear Factor kappa B (NF-κB) Pathway Downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqiang Cui,Yaping Lin,Yuhong Liu,Ling Cao,Li Cui

Nature communications 10:5410 PubMed31776338

2019

Thyroid hormone induces progression and invasiveness of squamous cell carcinomas by promoting a ZEB-1/E-cadherin switch.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Miro,Emery Di Cicco,Raffaele Ambrosio,Giuseppina Mancino,Daniela Di Girolamo,Annunziata Gaetana Cicatiello,Serena Sagliocchi,Annarita Nappi,Maria Angela De Stefano,Cristina Luongo,Dario Antonini,Feliciano Visconte,Silvia Varricchio,Gennaro Ilardi,Luigi Del Vecchio,Stefania Staibano,Anita Boelen,Cedric Blanpain,Caterina Missero,Domenico Salvatore,Monica Dentice

International immunopharmacology 76:105839 PubMed31520995

2019

MicroRNA-27a alleviates IL-1β-induced inflammatory response and articular cartilage degradation via TLR4/NF-κB signaling pathway in articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Jun Qiu,Ming-Ze Xu,Xiao-Dong Zhu,Yun-Han Ji

Cancer management and research 11:7439-7453 PubMed31496803

2019

Down-regulation of CCL17 in cancer-associated fibroblasts inhibits cell migration and invasion of breast cancer through ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Li,Chunli Yang,Jingshi Yang,Liqun Zou
View all publications
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