Human MMP13 ELISA Kit is a sandwich ELISA designed to quantify Human MMP13 with a sensitivity of 5.7 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 39 - 2500 pg/mL
View Alternative Names
Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13
- ELISA
Supplier Data
ELISA - Human MMP13 ELISA Kit (AB221839)
- ELISA
Supplier Data
ELISA - Human MMP13 ELISA Kit (AB221839)
Serum from ten individual healthy human donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-13 concentration was determined to be 32 pg/mL with a range of N.D. – 142 pg/mL.
- ELISA
Supplier Data
ELISA - Human MMP13 ELISA Kit (AB221839)
Example of human MMP-13 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- ELISA
Supplier Data
ELISA - Human MMP13 ELISA Kit (AB221839)
Interpolated concentrations of spiked MMP-13 in human serum, saliva, urine, and cell culture media samples.
The concentrations of MMP-13 were measured in duplicates, interpolated from the MMP-13 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, saliva 50%, urine 50%, and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Reactivity data
Product details
Human MMP13 ELISA Kit ab221839 is a rapid single-wash 90-min Sandwich ELISA to measure Human MMP13 in . This SimpleStep sensitivity is 5.7 pg/mL.
How the assay works
Human MMP13 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human MMP13 ELISA Kit ab221839 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-MMP13 antibody [EPR19120-143] - BSA and Azide free (Capture) ab242921
- Anti-MMP13 antibody [EPR19120-115] - BSA and Azide free (Detector) ab242921
Human MMP-13, matrix metallopeptidase 13, also known as Collagenase 3 is a secreted protein encoded by the gene MMP13. MMP-13 belongs to the matrix metalloproteinase family, which are calcium dependent endopeptidases. MMP-13 degrades type I, II, and III collagen. The MMP protein-13 is expressed as an inactive pro-form composed of an 83-amino acid pro-peptide, an amino acid catalytic domain, and hemopexin-like domain. Dissociation of a zinc ion from the cysteine-switch motif releases the activation-peptide, thus activating the enzyme. MMP-13 is involved in various processes, such as, embryonic development, ossification, wound healing, and tissue remolding.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.
Pathways
Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (3)
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Pharmaceutics 16: PubMed38543230
2024
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iScience 25:104645 PubMed35811850
2022
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Cytokine 131:155020 PubMed32413706
2020
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