Human MMP13 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MMP13 in Cow, Human, Pig in Saliva, Urine, Cell culture media, Serum samples.
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- iScience 25:1046452022Tumor cell-conditioned media drives collagen remodeling via fibroblast and pericyte activation in an premetastatic niche model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKylie G Nairon,Thomas J DePalma,Joshua M Zent,Jennifer L Leight,Aleksander SkardalPubMed 35811850
- Cytokine 131:1550202020Peptidomic analysis on synovial tissue reveals galectin-1 derived peptide as a potential bioactive molecule against rheumatoid arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunzheng Hu et. al.PubMed 32413706
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow, Human, Pig
- Range
- 39 - 2500 pg/mL
- Assay time
- 2h 30m
- Sensitivity
- = 5.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Alternative names
Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13
What's included?
Recommended products
Human MMP13 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human MMP13 in Cow, Human, Pig in Saliva, Urine, Cell culture media, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow, Human, Pig
- Range
- 39 - 2500 pg/mL
- Assay time
- 2h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 5.7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 5.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 83 | Range 78 - 88 % |
Sample type Saliva | Average % = 80 | Range 78 - 82 % |
Sample type Urine | Average % = 84 | Range 74 - 93 % |
Sample type Cell culture media | Average % = 102 | Range 101 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human MMP13 ELISA Kit (ab221839) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP13 protein in saliva, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP13 with 5.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human MMP-13, matrix metallopeptidase 13, also known as Collagenase 3 is a secreted protein encoded by the gene MMP13. MMP-13 belongs to the matrix metalloproteinase family, which are calcium dependent endopeptidases. MMP-13 degrades type I, II, and III collagen. The MMP protein-13 is expressed as an inactive pro-form composed of an 83-amino acid pro-peptide, an amino acid catalytic domain, and hemopexin-like domain. Dissociation of a zinc ion from the cysteine-switch motif releases the activation-peptide, thus activating the enzyme. MMP-13 is involved in various processes, such as, embryonic development, ossification, wound healing, and tissue remolding.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
- Biological function summary
Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.
- Pathways
Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.
- Associated diseases and disorders
Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.
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3 product images
ELISA - Human MMP13 ELISA Kit (ab221839)
Interpolated concentrations of spiked MMP-13 in human serum, saliva, urine, and cell culture media samples.
The concentrations of MMP-13 were measured in duplicates, interpolated from the MMP-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, saliva 50%, urine 50%, and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
ELISA - Human MMP13 ELISA Kit (ab221839)
Serum from ten individual healthy human donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-13 concentration was determined to be 32 pg/mL with a range of N.D. – 142 pg/mL.
ELISA - Human MMP13 ELISA Kit (ab221839)
Example of human MMP-13 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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