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AB100606

Human MMP2 ELISA Kit

5

(1 Review)

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(28 Publications)

Human MMP2 ELISA Kit is a sandwich ELISA designed to quantify Human MMP2 with a sensitivity of 3.5 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 3.3 - 800 ng/mL
- Cited in over 20 publications

View Alternative Names

CLG4A, MMP2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, TBE-1, MMP-2

2 Images
Sandwich ELISA - Human MMP2 ELISA Kit (AB100606)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP2 ELISA Kit (AB100606)

Representative Standard Curve using ab100606.

Sandwich ELISA - Human MMP2 ELISA Kit (AB100606)
  • sELISA

Lab

Sandwich ELISA - Human MMP2 ELISA Kit (AB100606)

MMP2 measured in biological fluids showing quantity (ng) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3.5 ng/mL

Range

3.3 - 800 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MMP2 ELISA Kit ab100606 is a sandwich ELISA to measure Human MMP2 in serum, plasma, cell culture supernatant with a sensitivity of 3.5 ng/ml.

How the assay works

This assay employs an antibody specific for Human MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human MMP2 ELISA Kit ab100606 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "69 - 92 %", "average": "= 77.25" }, { "sample": "Serum", "range": "70 - 93 %", "average": "= 81.27" }, { "sample": "Plasma", "range": "72 - 95 %", "average": "= 84.53" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100606-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100606-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100606-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100606-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MMP2 Microplate (12 x 8 wells)", "number":"AB100606-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100606-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human MMP2 Standard (lyophilized)", "number":"AB100606-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"440X HRP-Streptavidin Concentrate", "number":"AB100606-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human MMP2", "number":"AB100606-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
Biological function summary

Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.

Pathways

MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.

Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
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Product protocols

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Target data

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
See full target information MMP2
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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70512 PubMed40159633

2025

Clinical Significance of JAM-2 Expression in the Vaginal Wall Tissues of Patients With Pelvic Organ Prolapse.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Liu,Yajing Mao,Yuelin Wu,Sheng Wan,Shengyi Gu,Jing Peng,Bo Jiao,Xiaolin Hua

Medicina (Kaunas, Lithuania) 59: PubMed37109608

2023

Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yeldar Baiken,Zhannur Markhametova,Assem Ashimova,Ainur Zhulamanova,Assem Nogaibayeva,Larissa Kozina,Bakhyt Matkarimov,Bauyrzhan Aituov,Abduzhappar Gaipov,Askhat Myngbay

Cell cycle (Georgetown, Tex.) 21:2038-2050 PubMed35686740

2022

LncRNA LEMD1-AS1 relieves chondrocyte inflammation by targeting miR-944/PGAP1 in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Li,Kaihua Lian,Jianguang Mao,Fuguo Huang,Chunli Zhang,Jianguo Zang

Frontiers in oncology 12:918340 PubMed35747793

2022

Vitamin D Exerts Significant Antitumor Effects by Suppressing Vasculogenic Mimicry in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Khuloud Bajbouj,Abeer Al-Ali,Jasmin Shafarin,Lina Sahnoon,Ahmad Sawan,Ahmed Shehada,Walaaeldin Elkhalifa,Maha Saber-Ayad,Jibran Sualeh Muhammad,Adel B Elmoselhi,Salman Y Guraya,Mawieh Hamad

International journal of molecular sciences 23: PubMed35563650

2022

Enhancement of Farnesoid X Receptor Inhibits Migration, Adhesion and Angiogenesis through Proteasome Degradation and VEGF Reduction in Bladder Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Rui Lai,Hisao-Hsien Wang,Hsin-Han Chang,Yu-Ling Tsai,Wen-Chiuan Tsai,Chen-Ray Lee,Chih-Ying Changchien,Yu-Chen Cheng,Sheng-Tang Wu,Ying Chen

Cell stem cell 29:810-825.e8 PubMed35523141

2022

Stem-cell-derived trophoblast organoids model human placental development and susceptibility to emerging pathogens.

Applications

Unspecified application

Species

Unspecified reactive species

Rowan M Karvas,Shafqat A Khan,Sonam Verma,Yan Yin,Devesha Kulkarni,Chen Dong,Kyoung-Mi Park,Brian Chew,Eshan Sane,Laura A Fischer,Deepak Kumar,Liang Ma,Adrianus C M Boon,Sabine Dietmann,Indira U Mysorekar,Thorold W Theunissen

Small (Weinheim an der Bergstrasse, Germany) 17:e2007425 PubMed33690979

2021

Bioengineered Multicellular Liver Microtissues for Modeling Advanced Hepatic Fibrosis Driven Through Non-Alcoholic Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Jong Cho,Han-Jun Kim,KangJu Lee,Soufian Lasli,Aly Ung,Tyler Hoffman,Rohollah Nasiri,Praveen Bandaru,Samad Ahadian,Mehmet R Dokmeci,Junmin Lee,Ali Khademhosseini

Oncology letters 21:314 PubMed33692846

2021

Rap2B promotes the proliferation and migration of human glioma cells via activation of the ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guohong Shi,Zhen Zhang

Medicine 99:e20944 PubMed32629699

2020

Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Yang,Shuai Wang,Weihua Yu,Yixiong Zheng,Yulian Wu

BioMed research international 2020:5691671 PubMed32420351

2020

RAB9A Plays an Oncogenic Role in Human Liver Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Sun,Lei Li,Zhongchao Li
View all publications
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