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AB269371

Human MMP3 ELISA Kit

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(4 Publications)

Human MMP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MMP3 with a sensitivity of 4.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3

7 Images
ELISA - Human MMP3 ELISA Kit (AB269371)
  • ELISA

Supplier Data

ELISA - Human MMP3 ELISA Kit (AB269371)
Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)

Interpolated concentrations of native MMP3 in human MG-63 cell supernatant samples.

The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : MG-63 cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 1.2 ng/mL in MG-63 cell supernatant.

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)

Human MMP3 standard curve comparison.

Standard Curve comparison between human MMP3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)
  • sELISA

Unknown

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)

Interpolated concentrations of native MMP3 in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin) samples.

The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25%, plasma (EDTA) 17.5%, plasma (citrate) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 15.3 ng/mL in serum, 7.8 ng/mL in plasma (EDTA), 11.0 ng/mL in plasma (citrate) and 10.4 ng/mL in plasma (heparin).

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)

Human MMP3 standard curve comparison.

Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab189572) and current human MMP3 SimpleStep ELISA kit (ab269371).

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)

Serum from eight individual healthy MMP3 gender donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 9.53 ng/mL with a range of 3.6 – 15.5 ng/mL.

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP3 ELISA Kit (AB269371)

Example of human MMP3 standard curve in Sample Diluent NS.

The MMP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.4 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MMP3 ELISA Kit ab269371 is a rapid single-wash 90-min Sandwich ELISA to measure Human MMP3 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 4.4 pg/mL.

How the assay works

Human MMP3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MMP3 ELISA Kit ab269371 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP3 antibody [EPR22308-10] - BSA and Azide free (Capture) ab269405
- Anti-MMP3 antibody [EPR22308-55] - BSA and Azide free (Detector) ab269405

Matrix metalloprotease-3 (MMP3), also known as Stromelysin-1, is a 54 kD secreted protease involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP3 functions by degrading fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. This also activates pro-collagenase to further help in the remodeling of the extracellular matrix environment.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "111 - 116 %", "average": "= 114" }, { "sample": "Serum", "range": "107 - 115 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "104 - 118 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "107 - 111 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "101 - 120 %", "average": "= 110" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269371-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269371-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269371-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269371-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269371-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269371-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MMP3 Capture Antibody", "number":"AB269371-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB269371-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MMP3 Lyophilized Recombinant Protein", "number":"AB269371-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269371-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human MMP3 Detector Antibody (Lyophilized)", "number":"AB269371-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
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Product protocols

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Target data

Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed : 11029580, PubMed : 1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed : 2383557). Acts also intracellularly (PubMed : 22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed : 21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed : 35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed : 35940311).
See full target information MMP3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:7467-7479 PubMed39822505

2025

Effect of warm needle therapy guided by ultrasound on pain relief and improvement of physical function in patients with knee osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yuan,Ping Li

Heliyon 9:e20771 PubMed37842584

2023

Electroacupuncture combined with extracorporeal shock wave therapy improves pain symptoms and inflammatory factor levels in knee osteoarthritis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Shengfu Liu,Qiudan Chen,Qinggang Zhang,Kun Tao,Changhong Li,Baolei Chang,Weifeng Wang,Zhong Wu

Bioengineered 13:3240-3250 PubMed35045800

2022

Long non-coding RNA cancer susceptibility candidate 2 regulates the function of human fibroblast-like synoviocytes via the microRNA-18a-5p/B-cell translocation gene 3 signaling axis in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqin Ye,Lu Wei,Xietian Yin,Huiling Li,Guifu Qin,Siqi Li,Tingting Peng,Bo Liu,Shichao Zhao,Qin Zhuo

Molecular medicine reports 22:5463-5471 PubMed33174050

2020

Sulforaphane suppresses polyinosinic‑polycytidylic acid‑stimulated release of cytokines, chemokines and MMPs by human corneal fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Liu,Ye Liu,Hui Zheng,Xiaoshuo Zheng,Xiuxia Yang,Xiaojing Zhao,Lin Chen,Yang Liu
View all publications
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