Human MMP8 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(3 Publications )
- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327313
- sELISA
Supplier Data
Sandwich ELISA - Human MMP8 ELISA Kit (AB219050)
Interpolated concentrations of native MMP8 in human saliva and synovial fluid samples.
The concentrations of MMP8 were measured in duplicate, interpolated from the MMP8 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 37.5%, synovial fluid 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP8 concentrations were determined to be 15.4 ng/mL in saliva and 19.2 ng/mL in synovial fluid.
- sELISA
Supplier Data
Sandwich ELISA - Human MMP8 ELISA Kit (AB219050)
Interpolated concentrations of spiked MMP8 in human serum, plasma (citrate), plasma (EDTA), and plasma (heparin) samples.
The concentrations of MMP8 were measured in duplicate, interpolated from the MMP8 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1.25%, plasma (citrate) 1.25%, plasma (EDTA) 1.25%, plasma (heparin) 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Native signal was undetectable in null-spiked samples.
- ELISA
Supplier Data
ELISA - Human MMP8 ELISA Kit (AB219050)
Human MMP8 standard curve comparison data.
Standard curve comparison between human MMP8 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human MMP8 ELISA Kit (AB219050)
Example of human MMP8 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Human MMP8 ELISA Kit (ab219050) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP8 protein in cit plasma, edta plasma, hep plasma, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP8 with 19.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Matrix metalloproteinase-8 (MMP8), also known as neutrophil collagenase and PMNL collagenase, is a zinc and calcium binding protein encoded by the gene MMP-8. MMP8 belongs to the matrix metalloproteinase family which are involved in the degradation of extracellular matrix, and are involved in embryonic development, reproduction, and tissue remolding. MMP8 is stored inactive in granules within neutrophils, and upon secretion is activated by cleavage of a peptide through the dissociation of a conserved cysteine from a zinc ion. MMP8 degrades type I, II, and III collagens, and the protein contains four hemopexin regions.
Precision & recovery information
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" } ]
Recovery
[ { "sample": "Serum", "range": "81 - 86 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "79 - 85 %", "average": "= 82" }, { "sample": "Saliva", "range": "86 - 114 %", "average": "= 99.7" }, { "sample": "Heparin Plasma", "range": "82 - 90 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "83 - 87 %", "average": "= 85" } ]
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.
Pathways
MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Alternative Names
Publications (3)
Recent publications for all applications. Explore the full listand refine your search
Frontiers in medicine 9:814890 PubMed35145983
2022
Applications
Unspecified application
Species
Unspecified reactive species
BMC oral health 21:385 PubMed34353321
2021
Applications
Unspecified application
Species
Unspecified reactive species
Atherosclerosis 286:88-96 PubMed31103880
2019
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com