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AB246539

Human MMP9 ELISA Kit

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(1 Review)

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(23 Publications)

Human MMP9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MMP9 with a sensitivity of 22.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 20 citations

View Alternative Names

CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

7 Images
ELISA - Human MMP9 ELISA Kit (AB246539)
  • ELISA

Supplier Data

ELISA - Human MMP9 ELISA Kit (AB246539)
Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)

Interpolated concentrations of native MMP9 in human lymph node tissue based on a 100 μg/mL extract load.

The concentrations of MMP9 were measured in duplicate and interpolated from the MMP9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 896.33 pg/mL in lymph node tissue extract.

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)

Example of human MMP9 standard curve in 1X Cell Extraction Buffer PTR.

The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)

Example of human MMP9 standard curve in Sample Diluent NS.

The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)

10% serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 60.21 ng/mL with a range of 43.33 – 92.95 ng/mL.

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)

Human MMP9 Standard Curve Comparison.

Standard Curve comparison between human MMP9 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit (AB246539)

Interpolated concentrations of native MMP9 in human serum, plasma and cell culture supernatant samples.

The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (heparin) 6.25%, saliva 6.25%, 1.5% PHA-M stimulated cell culture supernatant 5%, and unstimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 64.45 ng/mL in serum, 138.41 ng/mL in plasma (heparin) and 159.25 ng/mL in saliva, 167.62 ng/mL in 1.5% PHA-M stimulated cell culture supernatant, and 8.70 ng/mL in unstimulated PBMC cell culture supernatant.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Cell culture extracts, Tissue Extracts, Heparin Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 22.17 pg/mL

Range

105.47 - 6750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human MMP9 ELISA Kit ab246539 is a rapid single-wash 90-min Sandwich ELISA to measure Human MMP9 in cell culture extracts, cell culture supernatant, heparin plasma, saliva, serum, tissue extracts. This SimpleStep sensitivity is 22.17 pg/mL.

How the assay works

Human MMP9 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MMP9 ELISA Kit ab246539 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP9 antibody [EPR18543-47] - BSA and Azide free (Capture) ab242979
- Anti-MMP9 antibody [EPR18543-172] - BSA and Azide free (Detector) ab242979

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 113 %", "average": "= 109" }, { "sample": "Serum", "range": "99 - 106 %", "average": "= 103" }, { "sample": "Saliva", "range": "103 - 108 %", "average": "= 106" }, { "sample": "Tissue Extracts", "range": "88 - 108 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "98 - 107 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
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Product protocols

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Target data

The protein expressed by gene MMP9 is a matrix metalloproteinase that plays an essential role in the local proteolysis of the extracellular matrix and in leukocyte migration. It cleaves KiSS1 at a Gly-|-Leu bond and processes NINJ1 to generate the Secreted ninjurin-1 form. Additionally, MMP9 cleaves type IV and type V collagen into large C-terminal three-quarter fragments and shorter N-terminal one-quarter fragments. It degrades fibronectin but not laminin or Pz-peptide. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MMP9
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed38927463

2024

Zinc Iodide Dimethyl Sulfoxide Reduces Collagen Deposition by Increased Matrix Metalloproteinase-2 Expression and Activity in Lung Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Roth,Bo Han,Chong Teck S'ng,Ba Xuan Hoang,Christopher Lambers

Biochemistry and biophysics reports 38:101690 PubMed38571555

2024

Glioblastoma U-87 cell electrotaxis is hindered by doxycycline with a concomitant reduction in the matrix metallopeptidase-9 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Fang Chang,Ji-Yen Cheng

EMBO molecular medicine 16:870-884 PubMed38462666

2024

Antiviral drugs prolong survival in murine recessive dystrophic epidermolysis bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Tartaglia,Ignacia Fuentes,Neil Patel,Abigail Varughese,Lauren E Israel,Pyung Hun Park,Michael H Alexander,Shiv Poojan,Qingqing Cao,Brenda Solomon,Zachary M Padron,Jonathan A Dyer,Jemima E Mellerio,John A McGrath,Francis Palisson,Julio Salas-Alanis,Lin Han,Andrew P South

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 24:406-418 PubMed38379280

2024

Human leukocyte antigen class I antibody-activated endothelium promotes CD206+ M2 macrophage polarization and MMP9 secretion through TLR4 signaling and P-selectin in a model of antibody-mediated rejection and allograft vasculopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Nevarez-Mejia,Yi-Ping Jin,Harry Pickering,Rajesh Parmar,Nicole M Valenzuela,Rebecca A Sosa,Sebastiaan Heidt,Gregory A Fishbein,Enrique Rozengurt,William M Baldwin,Robert L Fairchild,Elaine F Reed

iScience 26:107673 PubMed37705956

2023

LncRNA THUMPD3-AS1 promotes invasion and EMT in gastric cancer by regulating the miR-1297/BCAT1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zaibo Zhang,Yong Li,Liqiao Fan,Bingyu Wang,Wenbo Liu,Jiaxiang Cui,Bibo Tan

Frontiers in bioengineering and biotechnology 11:1215438 PubMed37545886

2023

Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals.

Applications

Unspecified application

Species

Unspecified reactive species

Bruna Natália Alves da Silva Pimentel,Sarah Raquel De Annunzio,Marcelo Assis,Paula Aboud Barbugli,Elson Longo,Carlos Eduardo Vergani

International journal of molecular sciences 24: PubMed37511457

2023

The Synergistic Effect of Electrical Stimulation and Dermal Fibroblast Cells-Laden 3D Conductive Hydrogel for Full-Thickness Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Hong Lin,En-Wei Liu,Yun-Jhen Lin,Hooi Yee Ng,Jian-Jr Lee,Tuan-Ti Hsu

Scientific reports 12:15347 PubMed36096917

2022

Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaoxia He,Zhongling Zheng,Tingting Liu,Yupei Ao,Yixuan Yang,Huaidong Hu

Journal of molecular medicine (Berlin, Germany) 100:1209-1221 PubMed35840740

2022

MiR-30c-1-3p targets matrix metalloproteinase 9 involved in the rupture of abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Hong-Gang Sui,Meng-Meng Wang,Jia-Yin Li,Xiao-Feng He,Jing-Yuan Li,Xiao-Zeng Wang

iScience 25:104645 PubMed35811850

2022

Tumor cell-conditioned media drives collagen remodeling via fibroblast and pericyte activation in an premetastatic niche model.

Applications

Unspecified application

Species

Unspecified reactive species

Kylie G Nairon,Thomas J DePalma,Joshua M Zent,Jennifer L Leight,Aleksander Skardal
View all publications
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