Human MMP9 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MMP9 in Saliva, Cell culture extracts, Tissue Extracts, Heparin Plasma, Cell culture supernatant, Serum samples.
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Publications
- iScience 26:1076732023LncRNA THUMPD3-AS1 promotes invasion and EMT in gastric cancer by regulating the miR-1297/BCAT1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZaibo Zhang,Yong Li,Liqiao Fan,Bingyu Wang,Wenbo Liu,Jiaxiang Cui,Bibo TanPubMed 37705956
- International journal of molecular sciences 24:2023The Synergistic Effect of Electrical Stimulation and Dermal Fibroblast Cells-Laden 3D Conductive Hydrogel for Full-Thickness Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYen-Hong Lin,En-Wei Liu,Yun-Jhen Lin,Hooi Yee Ng,Jian-Jr Lee,Tuan-Ti HsuPubMed 37511457
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Cell culture extracts, Tissue Extracts, Heparin Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
105.47 - 6750 pg/mL
- Assay time
1h 30m
- Sensitivity
= 22.17 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, PubMed:1480034, PubMed:12879005). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide.
Alternative names
Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, MMP9, CLG4B
What's included?
Recommended products
Human MMP9 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human MMP9 in Saliva, Cell culture extracts, Tissue Extracts, Heparin Plasma, Cell culture supernatant, Serum samples.
Alternative names
Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, MMP9, CLG4B
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Cell culture extracts, Tissue Extracts, Heparin Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
105.47 - 6750 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 22.17 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 109 | Range 103 - 113 % |
Sample type Serum | Average % = 103 | Range 99 - 106 % |
Sample type Saliva | Average % = 106 | Range 103 - 108 % |
Sample type Tissue Extracts | Average % = 95 | Range 88 - 108 % |
Sample type Heparin Plasma | Average % = 102 | Range 98 - 107 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human MMP9 ELISA Kit (ab246539) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP9 protein in hep plasma, saliva, serum, cell culture extracts, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP9 with 22.17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- Activity summary
The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
- Biological function summary
Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.
- Pathways
MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.
- Associated diseases and disorders
Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
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6 product images
Sandwich ELISA - Human MMP9 ELISA Kit (ab246539)
Human MMP9 Standard Curve Comparison.
Standard Curve comparison between human MMP9 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human MMP9 ELISA Kit (ab246539)
Example of human MMP9 standard curve in Sample Diluent NS.
The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP9 ELISA Kit (ab246539)
Example of human MMP9 standard curve in 1X Cell Extraction Buffer PTR.
The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MMP9 ELISA Kit (ab246539)
Interpolated concentrations of native MMP9 in human serum, plasma and cell culture supernatant samples.
The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (heparin) 6.25%, saliva 6.25%, 1.5% PHA-M stimulated cell culture supernatant 5%, and unstimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 64.45 ng/mL in serum, 138.41 ng/mL in plasma (heparin) and 159.25 ng/mL in saliva, 167.62 ng/mL in 1.5% PHA-M stimulated cell culture supernatant, and 8.70 ng/mL in unstimulated PBMC cell culture supernatant.
Sandwich ELISA - Human MMP9 ELISA Kit (ab246539)
Interpolated concentrations of native MMP9 in human lymph node tissue based on a 100 μg/mL extract load.
The concentrations of MMP9 were measured in duplicate and interpolated from the MMP9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 896.33 pg/mL in lymph node tissue extract.
Sandwich ELISA - Human MMP9 ELISA Kit (ab246539)
10% serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 60.21 ng/mL with a range of 43.33 – 92.95 ng/mL.
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