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AB327048

Human MMP9 ELISA Kit, Chemiluminescent

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Human MMP9 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human MMP9 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 13.72 - 30000 pg/ml with a sensitivity of 4.77 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
5 Images
Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)

Interpolated concentrations of native MMP9 in human serum plasma and cell culture supernatant samples.

The concentrations of MMP9 were measured in duplicates interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5% plasma (heparin) 6.25% saliva 6.25% 1.5% PHA-M stimulated cell culture supernatant 5% and unstimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MMP9 concentration was determined to be 64.45 ng/mL in serum 138.41 ng/mL in plasma (heparin) and 159.25 ng/mL in saliva 167.62 ng/mL in 1.5% PHA-M stimulated cell culture supernatant and 8.70 ng/mL in unstimulated PBMC cell culture supernatant.

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)

Interpolated concentrations of native MMP9 in human lymph node tissue based on a 100 µg/mL extract load.

The concentrations of MMP9 were measured in duplicate and interpolated from the MMP9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MMP9 concentration was determined to be 896.33 pg/mL in lymph node tissue extract.

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)

10% serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MMP9 concentration was determined to be 60.21 ng/mL with a range of 43.33 – 92.95 ng/mL.

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)
  • sELISA

Lab

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)

Standard curve comparison between the Chemiluminescent Human MMP9 SimpleStep ELISA and colorimetric Human MMP9 SimpleStep ELISA (colorimetric ab246539) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)
  • sELISA

Lab

Sandwich ELISA - Human MMP9 ELISA Kit, Chemiluminescent (AB327048)

Dynamic range comparison between the Chemiluminescent Human MMP9 SimpleStep ELISA, colorimetric Human MMP9 SimpleStep ELISA (colorimetric ab246539), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, Saliva, Tissue Extracts, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.77 pg/mL

Range

13.72 - 30000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human MMP9 ELISA Kit, Chemiluminescent (ab327048) is a rapid single-wash sandwich 55-min ELISA to measure Human MMP9 in Cell culture supernatant, Serum, Saliva, Tissue Extracts, Heparin Plasma with a sensitivity of 4.77 pg/mL.

How the assay works

Human MMP9 ELISA Kit, Chemiluminescent (ab327048) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MMP9 (ab327048) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP9 antibody [EPR18543-47] - BSA and Azide free (Capture) ab242950
- Anti-MMP9 antibody [EPR18543-172] - BSA and Azide free (Detector) ab242979

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 113 %", "average": "= 109" }, { "sample": "Serum", "range": "99 - 106 %", "average": "= 103" }, { "sample": "Saliva", "range": "103 - 108 %", "average": "= 106" }, { "sample": "Tissue Extracts", "range": "88 - 108 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "98 - 107 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed : 12879005, PubMed : 1480034, PubMed : 2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed : 12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed : 32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed : 1480034). Degrades fibronectin but not laminin or Pz-peptide.
See full target information MMP9

Alternative Names

CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com