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AB100610

Human MMP9 ELISA Kit (lyophilized)

5

(2 Reviews)

|

(46 Publications)

Human MMP9 ELISA Kit (lyophilized) is a Sandwich (quantitative) ELISA for the measurement of Human MMP9 (lyophilized) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Contactin-1, Neural cell recognition molecule F11, CNTN1

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Sandwich ELISA - Human MMP9 ELISA Kit (lyophilized) (AB100610)
  • sELISA

Unknown

Sandwich ELISA - Human MMP9 ELISA Kit (lyophilized) (AB100610)

MMP9 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).

Sandwich ELISA - Human MMP9 ELISA Kit (lyophilized) (AB100610)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP9 ELISA Kit (lyophilized) (AB100610)

Representative Standard Curve using ab100610.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MMP9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP9 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernatants.

This assay employs an antibody specific for Human MMP9 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 95.38" }, { "sample": "Serum", "range": "84 - 103 %", "average": "= 96.23" }, { "sample": "Plasma", "range": "83 - 102 %", "average": "= 94.64" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100610-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100610-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100610-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MMP9 Microplate (12 x 8 wells)", "number":"AB100610-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human MMP9", "number":"AB100610-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100610-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100610-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100610-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human MMP9 Standard (lyophilized)", "number":"AB100610-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
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Product protocols

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Target data

Mediates cell surface interactions during nervous system development. Interaction with TNR enhances the neurite outgrowth.
See full target information CNTN1
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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:1904 PubMed38253627

2024

Wound healing and photoprotection properties of Acanthus ebracteatus Vahl. extracts standardized in verbascoside.

Applications

Unspecified application

Species

Unspecified reactive species

Mayuree Kanlayavattanakul,Mattaka Khongkow,Nattaya Lourith

Medicina (Kaunas, Lithuania) 59: PubMed37109608

2023

Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yeldar Baiken,Zhannur Markhametova,Assem Ashimova,Ainur Zhulamanova,Assem Nogaibayeva,Larissa Kozina,Bakhyt Matkarimov,Bauyrzhan Aituov,Abduzhappar Gaipov,Askhat Myngbay

Science (New York, N.Y.) 379:eabn8934 PubMed36701450

2023

Low protease activity in B cell follicles promotes retention of intact antigens after immunization.

Applications

Unspecified application

Species

Unspecified reactive species

Aereas Aung,Ang Cui,Laura Maiorino,Ava P Amini,Justin R Gregory,Maurice Bukenya,Yiming Zhang,Heya Lee,Christopher A Cottrell,Duncan M Morgan,Murillo Silva,Heikyung Suh,Jesse D Kirkpatrick,Parastoo Amlashi,Tanaka Remba,Leah M Froehle,Shuhao Xiao,Wuhbet Abraham,Josetta Adams,J Christopher Love,Phillip Huyett,Douglas S Kwon,Nir Hacohen,William R Schief,Sangeeta N Bhatia,Darrell J Irvine

International journal of molecular sciences 23: PubMed36498929

2022

An Exploratory Study Provides Insights into MMP9 and Aβ Levels in the Vitreous and Blood across Different Ages and in a Subset of AMD Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Savannah A Lynn,Flavie Soubigou,Jennifer M Dewing,Amanda Smith,Joanna Ballingall,Thea Sass,Isabela Nica,Catrin Watkins,Bhaskar Gupta,Hussein Almuhtaseb,Stephen C Lash,Ho Ming Yuen,Angela Cree,Tracey A Newman,Andrew J Lotery,J Arjuna Ratnayaka

Current research in toxicology 3:100088 PubMed36176311

2022

Lactobacilli metabolites restore E-cadherin and suppress MMP9 in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Krupali Pawar,Clara Aranha

Journal of oncology 2022:8319221 PubMed35847358

2022

Effects of Surgery Combined with Different Chemotherapy on Matrix Metalloproteinase-9 and Tissue Inhibitors of Metalloproteinase-1 in Children with Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zilong Qi,Xinning Wang,Liang Yin,Kun Ma,Lei Chen,Jinmin Li

Frontiers in oncology 12:918340 PubMed35747793

2022

Vitamin D Exerts Significant Antitumor Effects by Suppressing Vasculogenic Mimicry in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Khuloud Bajbouj,Abeer Al-Ali,Jasmin Shafarin,Lina Sahnoon,Ahmad Sawan,Ahmed Shehada,Walaaeldin Elkhalifa,Maha Saber-Ayad,Jibran Sualeh Muhammad,Adel B Elmoselhi,Salman Y Guraya,Mawieh Hamad

Biomedicines 10: PubMed35625931

2022

Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlei Sun,Fujie Shi,Weiran Wang,Yunfei Wu,Shuang Qu,Jing Li,Hongwei Liang,Ke Zen

Pharmaceuticals (Basel, Switzerland) 15: PubMed35215366

2022

Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation.

Applications

Unspecified application

Species

Unspecified reactive species

Waleewan Eaknai,Phichaporn Bunwatcharaphansakun,Chutikorn Phungbun,Angkana Jantimaporn,Sasikan Chaisri,Suwimon Boonrungsiman,Ubonthip Nimmannit,Mattaka Khongkow

Toxins 13: PubMed34822613

2021

Crotoxin Modulates Events Involved in Epithelial-Mesenchymal Transition in 3D Spheroid Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ellen Emi Kato,Sandra Coccuzzo Sampaio
View all publications
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