AB324503

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular

SimpleStep
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Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular is a Sandwich (quantitative) ELISA for the measurement of Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) - Extracellular in Human, Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1

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Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Example of human/mouse alpha 1 Sodium Potassium ATPase standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Recombinant alpha 1 Sodium Potassium ATPase was spiked into the following mouse samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and cell culture media (DMEM with 10% FBS). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Specificity and interference testing of human/mouse alpha 1 Sodium Potassium ATPase SimpleStep ELISA kit. 2,500 pg/mL of human (Hu) ATP1A1 and mouse (Ms) ATP1A1 were tested individually for species reactivity. 50 ng/mL each of recombinant mouse (Ms) ATP1A2, ATP1A3, and ATP1A4 were individually tested for specificity, and for interference in combination with 2,500 pg/mL of recombinant mouse ATP1A1. The interpolated values, expressed in % of input (for specificity) or in % of mouse ATP1A1 control (for interference), are plotted (mean +/- SD, n =2). No cross-reactivity or interference was observed with ATP1A2, ATP1A3, and ATP1A4.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Recombinant alpha 1 Sodium Potassium ATPase was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer. Undiluted samples are 50% serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

Example of human/mouse alpha 1 Sodium Potassium ATPase standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Human, Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 46.942 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) with a sensitivity of 46.942pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.2" } ]

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Recovery

[ { "sample": "Serum", "range": "79 - 93 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "84 - 110 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "82 - 98 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "90 - 109 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (PubMed : 17408578).

See full target information Atp1a1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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