AB324504

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular

SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA for the measurement of Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) - Intracellular in Human, Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1

5 Images

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular (AB324504)

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular (AB324504)

Interpolated concentration of native alpha 1 Sodium Potassium ATPase was measured in duplicate at different sample concentrations in human cortex tissue extract in 96-well vs. 384-well plates. Undiluted samples are human cortex tissue extract 10 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular (AB324504)

Example of human/mouse alpha 1 Sodium Potassium ATPase standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular (AB324504)

Specificity and interference testing of human/mouse alpha 1 Sodium Potassium ATPase SimpleStep ELISA kit. 2,500 pg/mL of human (Hu) ATP1A1and Mouse (Ms) ATP1A1 were tested individually for species reactivity. 50 ng/mL each of recombinant mouse (Ms) ATP1A2, ATP1A3, and ATP1A4 were individually tested for specificity, and for interference in combination with 2,500 pg/mL of recombinant mouse ATP1A1. The interpolated values, expressed in % of input (for specificity) or in % of mouse ATP1A1 control (for interference), are plotted (mean +/- SD, n =2). No cross-reactivity or interference was observed with ATP1A2, ATP1A3, and ATP1A4.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular (AB324504)

Example of human/mouse alpha 1 Sodium Potassium ATPase standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Intracellular (AB324504)

Interpolated concentration of native alpha 1 Sodium Potassium ATPase was measured in duplicate at different sample concentrations. Undiluted samples are as follows : RAW264.7 cell extract 50 ug/mL, SH-SY5Y cell extract 500 ug/mL, mouse brain tissue extract 20 ug/mL, and human cortex tissue extract 10 ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Key facts

Detection method

Colorimetric

Sample types

Cell Extract, Tissue Extracts

Reacts with

Human, Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 49.093 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) with a sensitivity of 49.093pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.2" } ]

style

left-column

Recovery

[ { "sample": "Cell Lysate", "range": "86 - 108 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "88 - 122 %", "average": "= 107" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab324504-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB324504-CMP10" }, { "size":"10 x 600 µL", "name":"10X Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Detector Antibody", "number":"AB324504-CMP02" }, { "size":"20 x 1 Vial", "name":"Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Lyophilized Recombinant Protein", "number":"AB324504-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB324504-CMP13" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324504-CMP07" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324504-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB324504-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324504-CMP11" }, { "size":"10 x 600 µL", "name":"10X Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Capture Antibody", "number":"AB324504-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324504-CMP04" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324504-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab324504-1x384Tests", "assetComponentDetails": [ { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324504-CMP07" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB324504-CMP10" }, { "size":"1 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324504-CMP05" }, { "size":"2 x 1 Vial", "name":"Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Lyophilized Recombinant Protein", "number":"AB324504-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB324504-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Detector Antibody", "number":"AB324504-CMP02" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324504-CMP04" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB324504-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Capture Antibody", "number":"AB324504-CMP01" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324504-CMP13" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324504-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324504-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Detector Antibody", "number":"AB324504-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB324504-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB324504-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324504-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB324504-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324504-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB324504-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324504-CMP13", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324504-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Lyophilized Recombinant Protein", "number":"AB324504-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324504-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) Capture Antibody", "number":"AB324504-CMP01", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (PubMed : 17408578).

See full target information Atp1a1

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com