Human/Mouse Oct4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human/Mouse Oct4 in Cell culture extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Mouse
- Range
- 1.76 - 112.5 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 596 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Alternative names
OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3
What's included?
Recommended products
Human/Mouse Oct4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human/Mouse Oct4 in Cell culture extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human, Mouse
- Range
- 1.76 - 112.5 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 596 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 8 | mean - | SD - | C.V. 9.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 102 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Oct4 ELISA Kit (ab235653) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oct4 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human and mouse Oct4 with 596 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
- Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
- Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
- Associated diseases and disorders
Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.
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2 product images
Sandwich ELISA - Human/Mouse Oct4 ELISA Kit (ab235653)
Example of human Oct4 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human/Mouse Oct4 ELISA Kit (ab235653)
Interpolated concentrations of native Oct4 in NCCIT cell extract based on a 50 μg/mL extract load.
The concentrations of Oct4 were measured in duplicate and interpolated from the Oct4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Oct4 concentration was determined to be 26.7 ng/mL in NCCIT cell extract.
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