Human MR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MR1 with a sensitivity of 9.69 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.125 - 5000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 9.69 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells (PubMed:12794138, PubMed:19416870, PubMed:22692454, PubMed:23051753, PubMed:23846752, PubMed:26795251). In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1-2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers (PubMed:20581831, PubMed:24695216, PubMed:26795251). Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively (PubMed:24695216). May present microbial antigens to various TRAV1-2-negative MAIT cell subsets, providing for unique recognition of diverse microbes, including pathogens that do not synthesize riboflavin (PubMed:27527800, PubMed:31113973). Upon antigen recognition, elicits rapid innate-type MAIT cell activation to eliminate pathogenic microbes by directly killing infected cells (PubMed:23846752, PubMed:24695216, PubMed:27527800). During T cell development, drives thymic selection and post-thymic terminal differentiation of MAIT cells in a process dependent on commensal microflora (By similarity). Acts as an immune sensor of cancer cell metabolome (PubMed:31959982). May present a tumor-specific or -associated metabolite essential for cancer cell survival to a pan-cancer TCR consisting of TRAV38.2-DV8*TRAJ31 alpha chain paired with a TRBV25.1*TRBJ2.3 beta chain on a non-MAIT CD8-positive T cell clone (MC.7.G5), triggering T cell-mediated killing of a wide range of cancer cell types (PubMed:31959982).
Alternative names
Major histocompatibility complex class I-related gene protein, MHC class I-related gene protein, Class I histocompatibility antigen-like protein, MR1
What's included?
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Human MR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MR1 with a sensitivity of 9.69 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.125 - 5000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9.69 pg/mL
- General recovery
- 95%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 5.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 8.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 107 | Range 99 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human MR1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MR1 protein in Cell Lysate. Quantitate Human MR1 with 9.69 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MR1 also known as the MR1 receptor or MR1 protein is an important player in the immune system. It weighs around 37 kilodaltons. This protein expresses prominently in many tissues such as the liver lymphoid tissues and the intestine. MR1 binds a range of small molecules derived from vitamin B metabolites. It presents these metabolites to a specific group of immune cells known as mucosal-associated invariant T (MAIT) cells leading to their activation.
- Biological function summary
MR1 varies its mechanism by forming complexes with antigens that lack traditional peptide structure. It performs a major role in the immune response particularly through its interactions with MAIT cells. These cells represent an important intersection between innate and adaptive immunity responding rapidly to many pathogen-associated metabolites presented by MR1. This response contributes importantly to host protection especially against microbial infections.
- Pathways
MR1 is an integral component in the vitamin B antigen presentation pathway. It interacts significantly with other molecules involved in antigen presentation. MR1's function anchors it within the host-pathogen interaction pathways and connects with proteins like beta-2-microglobulin (β2M) which is important for surface expression. Its activity complements classical MHC pathways although it operates independently in presenting non-peptide antigens.
- Associated diseases and disorders
MR1 contributes to understanding certain autoimmune conditions and infectious diseases. It connects to infectious diseases such as tuberculosis where MAIT cell activation plays a critical role in the immune response. Additionally MR1 has links to inflammatory bowel disease (IBD) a condition where gut microbiota activation of MAIT cells through MR1 may influence the disease outcome. Understanding its role in these diseases can provide insights into how MR1 or related proteins might be targeted for therapeutic interventions.
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2 product images
Sandwich ELISA - Human MR1 ELISA Kit (ab315070)
Example of human MR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human MR1 ELISA Kit (ab315070)
Interpolated concentration of native MR1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: THP-1 cell extract 500 µg/mL and K562 cell extract 1,500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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