JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272211

Human MRP1 ELISA Kit (ABCC1)

Be the first to review this product! Submit a review

|

(0 Publication)

Human MRP1 ELISA Kit (ABCC1) is a single-wash 90-min Simplestep used to quantify Human MRP1 (ABCC1) with a sensitivity of 0.151 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity

View Alternative Names

MRP, MRP1, ABCC1, Multidrug resistance-associated protein 1, ATP-binding cassette sub-family C member 1, Glutathione-S-conjugate-translocating ATPase ABCC1, Leukotriene C(4) transporter, LTC4 transporter

4 Images
Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)
  • sELISA

Supplier Data

Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)

Interpolated concentrations of native MRP1 in human A431 cell, HeLa cell and lung tissue based on 150 μg/mL, 600 µg/mL and 1,000 µg/mL extract loads, respectively.

The concentrations of MRP1 were measured in duplicate and interpolated from the MRP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MRP1 concentration was determined to be 13.6 ng/mL in A431 extract, 24.1 ng/mL in HeLa extract and 5.1 ng/mL in lung extract.

Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)
  • sELISA

Supplier Data

Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)

Representative image of Human MRP1 ELISA Kit (ABCC1) (ab272211)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human MRP1 ELISA Kit (ABCC1) Capture Antibody

- 10X Human MRP1 (ABCC1) Detector Antibody

- 10X Human MRP1 ELISA Kit (ABCC1) Lyophilised Purified Protein (2 vials)

- Human MRP1 ELISA Kit (ABCC1) 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)
  • sELISA

Supplier Data

Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)

Example of human MRP1 standard curve in 1X Cell Extraction Buffer PTR.

The MRP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)
  • sELISA

Supplier Data

Sandwich ELISA - Human MRP1 ELISA Kit (ABCC1) (AB272211)

Interpolated concentrations of native MRP1 in human control wild type HeLa cell and MRP1 knockout HeLa cell based on 200 μg/mL extract loads.

The concentrations of MRP1 were measured in duplicate and interpolated from the MRP1 standard curve and expressing the interpolated concentrations as ng of protein per mg of extract (mean +/- SD, n=2). The mean MRP1 concentration was determined to be 11.8 ng/mg in wild type HeLa extract and undetectable in MRP1 knockout HeLa extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.151 ng/mL

Range

0.63 - 40 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB214030

Human IL-8 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

  • 5
  • 2
Assay Kits

AB185986

Human IL-10 ELISA Kit (Interleukin-10)

KO Validated|Recombinant|SimpleStep

ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

  • 0
  • 0
Assay Kits

AB219631

Human EMMPRIN ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)

  • 0
  • 0
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human MRP1 ELISA kit (ab272211) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MRP1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MRP1 with 151 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MRP1 (Multidrug Resistance-associated Protein 1), also known as ABCC1 (ATP-Binding Cassette sub-family C member 1), mediates export of organic anions and drugs from the cytoplasm. It mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. MRP1 confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]
style
left-column

Recovery

[ { "sample": "Tissue Extracts", "range": "94 - 96 %", "average": "= 95" }, { "sample": "Cell Lysate", "range": "91 - 97 %", "average": "= 94" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272211-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272211-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272211-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272211-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB272211-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272211-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272211-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272211-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MRP1 (ABCC1) Lyophilized Recombinant Protein", "number":"AB272211-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MRP1 (ABCC1) Capture Antibody", "number":"AB272211-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB272211-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MRP1 (ABCC1) Detector Antibody", "number":"AB272211-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272211-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MRP1 also known as ABCC1 is a transporter protein with a mass of approximately 190 kDa. This protein belongs to the ATP-binding cassette (ABC) transporter family. MRP1 actively exports a variety of substrates from cells by hydrolyzing ATP to ADP and inorganic phosphate. You can find MRP1 expressed in many tissues including the lung testis and kidney. It helps in cellular detoxification by exporting organic anions and other conjugated metabolites.
Biological function summary

This protein functions as an important determinant of multidrug resistance in cancer treatment. MRP1 can form part of a larger protein complex that includes other transporters like MRP2. Through its activity MRP1 protects tissues from toxic substances by transporting them out of the cell. Its role in transporting glutathione-conjugated compounds highlights its importance in cellular defense mechanisms against oxidative stress and xenobiotics.

Pathways

MRP1 participates in the glutathione metabolism and the xenobiotic efflux pathways. Both pathways involve cellular detoxification and the accumulation of anomalies can cause harmful effects in the body. MRP1 works with proteins such as GSTP1 which conjugates toxic substances with glutathione preparing them for export by MRP1. This coordination ensures efficient detoxification and protection of cells from damage.

MRP1 has associations with several conditions particularly drug resistance in various cancers and chronic obstructive pulmonary disease (COPD). In cancer MRP1 overexpression often results in reduced treatment efficacy due to chemotherapy drugs being expelled from the cell helping to resist their cytotoxic effects. Research indicates a connection between MRP1 and P-glycoprotein (ABCB1) in cancer drug resistance pointing to a broader resistive mechanism in which multiple transporters are involved.
style
left-column

Product protocols

style
left-column

Target data

Mediates export of organic anions and drugs from the cytoplasm (PubMed : 10064732, PubMed : 11114332, PubMed : 16230346, PubMed : 7961706, PubMed : 9281595). Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics (PubMed : 10064732, PubMed : 11114332, PubMed : 16230346, PubMed : 7961706, PubMed : 9281595). Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export (PubMed : 9281595). Hydrolyzes ATP with low efficiency (PubMed : 16230346). Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (PubMed : 17050692). Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthesizing cells (By similarity). Mediates ATP-dependent, GSH-independent cyclic GMP-AMP (cGAMP) export (PubMed : 36070769). Thus, by limiting intracellular cGAMP concentrations negatively regulates the cGAS-STING pathway (PubMed : 36070769). Exports S-geranylgeranyl-glutathione (GGG) in lymphoid cells and stromal compartments of lymphoid organs. ABCC1 (via extracellular transport) with GGT5 (via GGG catabolism) establish GGG gradients within lymphoid tissues to position P2RY8-positive lymphocytes at germinal centers in lymphoid follicles and restrict their chemotactic transmigration from blood vessels to the bone marrow parenchyma (By similarity). Mediates basolateral export of GSH-conjugated R- and S-prostaglandin A2 diastereomers in polarized epithelial cells (PubMed : 9426231).
See full target information ABCC1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com