Human MS2 ELISA Kit

Product details

The Human MS2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213750) is designed for the quantitative measurement of Human MS2 in cell culture supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for MS2 has been pre-coated onto 96-well plates. Standards (NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MS2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MS2 amount of sample captured in plate.

MS2 (also known as ADAM8), a disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the MS2 /ADAM8 gene. MS2 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as MS2, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration.