JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB322369

Human mTOR ELISA Kit- Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human mTOR ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human mTOR with a sensitivity of 0.023 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, mTOR

2 Images
Sandwich ELISA - Human mTOR ELISA Kit- Extracellular (AB322369)
  • sELISA

Supplier Data

Sandwich ELISA - Human mTOR ELISA Kit- Extracellular (AB322369)

Example of human mTOR standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human mTOR ELISA Kit- Extracellular (AB322369)
  • sELISA

Supplier Data

Sandwich ELISA - Human mTOR ELISA Kit- Extracellular (AB322369)

Example of human mTOR standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.023 ng/mL

Range

0.266 - 17 ng/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human mTOR SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of mTOR protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Urine. Quantitate Human mTOR with 0.023 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 0.9" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "91 - 93 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "90 - 97 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "86 - 93 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "87 - 93 %", "average": "= 90" }, { "sample": "Cell culture media", "range": "98 - 101 %", "average": "= 100" }, { "sample": "Urine", "range": "89 - 92 %", "average": "= 90" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322369-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322369-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human mTOR Detector Antibody", "number":"AB322369-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human mTOR Capture Antibody", "number":"AB322369-CMP01" }, { "size":"20 x 1 Vial", "name":"Human mTOR Lyophilized Recombinant Protein", "number":"AB322369-CMP03" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322369-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322369-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322369-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322369-CMP10" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322369-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322369-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322369-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322369-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human mTOR Detector Antibody", "number":"AB322369-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human mTOR Capture Antibody", "number":"AB322369-CMP01" }, { "size":"2 x 1 Vial", "name":"Human mTOR Lyophilized Recombinant Protein", "number":"AB322369-CMP03" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322369-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322369-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322369-CMP10" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322369-CMP11" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322369-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322369-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322369-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322369-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human mTOR Detector Antibody", "number":"AB322369-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human mTOR Capture Antibody", "number":"AB322369-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human mTOR Lyophilized Recombinant Protein", "number":"AB322369-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322369-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322369-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322369-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322369-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322369-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322369-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

The protein expressed by the MTOR gene is a serine/threonine protein kinase that serves as a central regulator of cellular metabolism, growth, and survival in response to various signals, such as hormones and nutrients. MTOR operates within two distinct signaling complexes, mTORC1 and mTORC2. mTORC1 is activated to upregulate protein synthesis by phosphorylating regulators of mRNA translation and ribosome synthesis, and phosphorylates and activates proteins like RPS6KB1 and RPS6KB2 to promote protein synthesis. It controls MiT/TFE factors TFEB and TFE3 by mediating their retention and inactivation under nutrient-rich conditions, and it inhibits autophagy by phosphorylating DAP and RUBCNL/Pacer. Additionally, mTORC1 engages in feedback control on growth factor signaling and may influence microtubules through CLIP1 phosphorylation. The mTORC2 complex may regulate cellular processes, including survival and cytoskeletal organization, by phosphorylating AKT1 and regulating the actin cytoskeleton via PRKCA, PXN, and Rho-type guanine nucleotide exchange factors. It also regulates the phosphorylation of SGK1. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MTOR
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com